DNA Damage Quantification Colorimetric Kit

Description

Detects AP sites
Summary

• Detection method- Absorbance (450 and 650 nm) • Species reactivity- Mammalian • Applications- Kit provides all necessary reagents for convenient determination of DNA damage (abasic sites) in sample DNA
Sample Type

Purified damaged DNA
Features Benefits

• Kit provides all necessary reagents for 96-well plate format
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

Apurinic/apyrimidinic (AP) sites are one of the major types of DNA lesions formed during the course of base excision and repair of oxidized, deaminated or alkylated bases. It has been estimated that about 2x105 base lesions are generated per cell per day. The level of AP sites in cells can be a good indicator of DNA lesion and repair against chemical damage and cell aging. The DNA Damage Quantification Kit utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with an aldehyde group which is the open ring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using avidin-biotin assay followed by a colorimetric detection. The kit provides the necessary reagents for convenient determination of abasic sites in purified DNA sample in 96-well plate format.
Data Sheet Link
Properties

Colorimetric assays or detection use UV absorption or enzymatic color reaction.

Gene target

DNA Damage Quantification Kit
Gene symbol

PRKDC, HLA-DOA
Short name

DNA Damage Quantification Colorimetric Kit
Technique

colorimetric, dna, colorimetry
Alternative name

Desoxyribonucleic acid Damage Quantification colorimetry reagent
Alternative technique

assays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data