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DNA Damage Quantification Colorimetric Kit

  • Catalog number
    K253-25
  • Product name
    DNA Damage Quantification Colorimetric Kit
  • Size
    25 assays
  • Description
    Detects AP sites
  • Summary
    • Detection method- Absorbance (450 and 650 nm) • Species reactivity- Mammalian • Applications- Kit provides all necessary reagents for convenient determination of DNA damage (abasic sites) in sample DNA
  • Sample Type
    Purified damaged DNA
  • Features Benefits
    • Kit provides all necessary reagents for 96-well plate format
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Apurinic/apyrimidinic (AP) sites are one of the major types of DNA lesions formed during the course of base excision and repair of oxidized, deaminated or alkylated bases. It has been estimated that about 2x105 base lesions are generated per cell per day. The level of AP sites in cells can be a good indicator of DNA lesion and repair against chemical damage and cell aging. The DNA Damage Quantification Kit utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with an aldehyde group which is the open ring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using avidin-biotin assay followed by a colorimetric detection. The kit provides the necessary reagents for convenient determination of abasic sites in purified DNA sample in 96-well plate format.
  • Data Sheet Link
    Data_Sheet_Link
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    DNA Damage Quantification Kit
    • Gene info
    Locus Specific Databases:LRG_162
    Gene info
    Locus Specific Databases:IMGT/HLA Database
  • Gene symbol
    PRKDC, HLA-DOA
  • Short name
    DNA Damage Quantification Colorimetric Kit
  • technique filter
    • colorimetric
    • dna
  • Technique
    colorimetric, dna, colorimetry
  • Alternative technique
    assays, kits