Cathepsin D Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K148-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple Assay, HTS
• Summary
  • Detection method- Fluorescence (Ex/Em = 328/460 nm) • Kit size- 100 assays • Applications- Assay kit to detect putative Cathepsin D inhibitors
• Sample Type
  Purified Cathepsin D, Recombinant Cathepsin D
• Features Benefits
  • Simple one-step procedure; takes only 1 hours • Fast, robust and convenient • Cathepsin-D inhibitor screening assay is a fast and robust assay to screen potential inhibitors • Positive control inhibitor, Pepstatin A is included in the kit
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  6-12 months
• Background
  Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA. Cathepsin D will cleave the synthetic substrate to release the quenched fluorescent group MCA, which can then easily be measured using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm. The relative efficacy of test inhibitors are compared to the positive control inhibitor, Pepstatin A (IC50 < 0.1 nM). The Cathepsin D assay is simple, straightforward, and can be adapted to 96-well plate assays and is suitable for high throughput screening (HTS).
• Data Sheet Link
• Gene
  CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Cathepsin   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  Cathepsin D Inhibitor Screening Kit (Fluorometric)
• Alternative name
  Cathepsin D suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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