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Caspase-2 Fluorometric Assay Kit

  • Catalog number
    K116-25
  • Product name
    Caspase-2 Fluorometric Assay Kit
  • Size
    25 assays
  • Description
    Convenient, Highly-Sensitive Assay
  • Summary
    • Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 200, 400 assays) • Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  • Sample Type
    Cell and tissue lysates
  • Features Benefits
    • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-2 activity.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
  • Data Sheet Link
    Data_Sheet_Link
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Caspase-2 Fluorometric Kit
  • Host
    Assay
  • Short name
    Caspase-2 Fluorometric Assay Kit
  • technique filter
    • Assay
  • Technique
    Assay, assays
  • Alternative technique
    arrays, kits