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Caspase-10/a, human recombinant

  • Catalog number
    1090A-25
  • Product name
    Caspase-10/a, human recombinant
  • Size
    25 units
  • Synonyms
    Caspase-10/a
  • Alternative names
    Caspase-10, CASP-10, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2, ICE-like apoptotic protease 4
  • Description
    A cysteine protease involved in apoptosis
  • Recombinant
    Yes
  • Source
    E. coli.
  • Purity by SDS PAGE
    ≥90%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    > 8000 units/mg
  • Unit Definition
    One unit of the recombinant caspase-10/a is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
  • Molecular Weight
    two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form.
  • Storage Temp
    -70°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    Lyophilized powder
  • Reconstitution Instructions
    Reconstitute to 1 unit per ul in PBS containing 15% glycerol.
  • Background Information
    Caspase-10/a (also known as Mch4) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form. The recombinant active human caspase-10 was expressed in E. coli. The active caspase-10 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Datasheet
    Datasheet
  • Gene target
    Caspase-10/a
    • Gene info
  • Gene symbol
    MINDY3
  • Short name
    Caspase-10/a, recombinant
  • technique filter
    • Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Alternative technique
    rec