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Anti-Flavivirus group antigen Antibody

  • Catalog number
    A1147-200
  • Product name
    Anti-Flavivirus group antigen Antibody
  • Size
    200 µg
  • Antibody Type
    Monoclonal
  • Clone
    D1-4G2-4-15 (4G2)
  • Isotype
    Mouse Ig2a, kappa
  • Immunogen Sequence
    Dengue Virus type 2 antigens
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    200 µg affinity purified antibody in phosphate-buffered saline (PBS) containing 0.02% Proclin 300
  • Purification
    Affinity purified using protein A
  • Application
    IF; WB; NTRL; ELISA
  • Positive Control
    ELISA: Dengue Virus Serotype 2 VLPs
  • Description
    This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation. The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004. Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008).
  • Storage Condition
    -20°C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans
  • Handling
    The antibody solution should be gently mixed before use
  • Western Blot Verified
    Not Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Data Sheet Link
    Data_Sheet_Link
  • Gene target
    Flavivirus group
  • isotype filter
    • Mouse Ig2a
    • kappa
  • Short name
    Anti-Flavivirus group antigen Antibody
  • technique filter
    • Antibody
    • antigen
    • anti-
    • anti
  • Technique
    Antibody, antigen, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antigenes
  • Alternative technique
    antibodies, antigenes