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Antibody Target
CD80
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Target Alternative Name
B7-1; T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7
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Antibody Type
Monoclonal
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Clone
IDEC-114 (Galiximab)
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Species Reactivities
Human
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Immunogen Sequence
Galiximab was prepared by immunizing cynomolgus monkeys with recombinant CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line
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Accession
P33681
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Gene Id
941
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
1 mg/ml
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Formulation
In PBS with 0.02% Proclin 300
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Purification
Protein A affinity purified
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Application
Block, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting, Modulate, Flow Cytometry, ELISA
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Application And Usages
WB, ELISA, IP, IF, IHC, FC, Block, Modulate
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Description
Galiximab is a primatized mAb which consists of human constant and primate (cynomolgus macaque) variable regions and binds specifically to CD80. CD80 is a surface glycoprotein and a member of the B7 family of co-stimulatory molecules. CD80 antigen regulates T cell activation (through interacting with CD28 or CD152) and is expressed transiently in antigen-presenting cells, T cells and normal B cells, and expressed constitutively on various subtypes of B-cell lymphomas. Galiximab acts to decrease in cell proliferation, inhibition of the constitutively active NF-κB pathway, increase in apoptosis and ADCC against various B-cell lymphoma cell lines. Galiximab affects CD28 and CD152 interactions with CD80.
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Storage Condition
-20ºC
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Shipping Condition
Gel Pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
False
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Immunocytochemistry Verified
False
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Immunofluorescence Verified
True
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Immunoprecipitation Verified
False
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FACS Verified
False
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ELISA Verified
False
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ChIP Verified
False
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Dot Blot Verified
False
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Flow Cytometry Verified
False
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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French translation
anticorps
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Gene target
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Gene symbol
CD80
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Short name
Anti-CD80 (Galiximab), IgG1, lambda Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Isotype
Human IgG1, lambda, IgG1
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Species
Human, Humans
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Alternative name
Antibody toCD80 (Galiximab), H. sapiens IgG1, lambda (antibody to-)
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Alternative technique
antibodies
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Alternative to gene target
CD80 molecule, B7 and B7-1 and B7.1 and BB1 and CD28LG and CD28LG1 and LAB7, CD80 and IDBG-51287 and ENSG00000121594 and 941, coreceptor activity, Cell surfaces, Cd80 and IDBG-160288 and ENSMUSG00000075122 and 12519, BT.27986 and IDBG-632750 and ENSBTAG00000018059 and 407131
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