- Catalog numberbsm-51006M
- Product nameHIS Tag (6AT18) Monoclonal Antibody
- Size0.1ml
- PriceAsk For Price
- Short nameAnti-HIS Tag
- Long nameHIS Tag (6AT18) Monoclonal Antibody
- Also known asHIS Tag (6AT18) Monoclonal Antibody
- CategoryPrimary Antibodies
- ConjugationUnconjugated
- HostMouse (Mus musculus)
- Target AntigenHIS Tag
- SpecificityThis is a highly specific antibody against HIS Tag.
- Modification sNone
- Modification site sUnmodified antibody
- ClonalityMonoclonal
- Clone number6AT18
- IsotypeImmunoglobulin G (IgG)1
- SourcePurified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
- Tested ApplicationsWB
- Recommended dilutionsWB(1:1000-2000)
- Cross reactivityOthers
- Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
- Background of the target antigenEpitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
- Purification methodMouse monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
- Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
- Gene targetHIS Tag 6AT18 Monoclonal
- Identity:HGNC:4806
- Gene:HAL
- Long gene name:histidine ammonia-lyase
- Discovery year:1988-08-31
- Entrez gene record:3034
- VEGA ID:OTTHUMG00000170354
- Identity:HGNC:382
- Gene:AKR1B10
- Long gene name:aldo-keto reductase family 1 member B10
- Synonyms gene name:aldo-keto reductase family 1, member B10 (aldose reductase)
- Synonyms:AKR1B12ARL-1HISARL1HSIALDRLn
- Discovery year:2000-08-24
- Entrez gene record:57016
- Pubmed identification:9765596, 9565553
- RefSeq identity:NM_020299
- Classification:Aldo-keto reductases
- VEGA ID:OTTHUMG00000155356
- Identity:HGNC:34535
- Gene:TRH-GTG1-1
- Long gene name:tRNA-His (anticodon GTG) 1-1
- Synonyms gene name:transfer RNA histidine 4 (anticodon GUG), transfer RNA-His (GTG) 1-1
- Synonyms:tRNA-His-GTG-1-1,
- Discovery year:2008-08-21
- Entrez gene record:100188991
- Classification:Cytoplasmic transfer RNAs
- Identity:HGNC:34874
- Gene:TRH-GTG1-3
- Long gene name:tRNA-His (anticodon GTG) 1-3
- Synonyms gene name:transfer RNA histidine 9 (anticodon GUG), transfer RNA-His (GTG) 1-3
- Synonyms:tRNA-His-GTG-1-3,
- Discovery year:2008-08-29
- Entrez gene record:100189307
- Classification:Cytoplasmic transfer RNAs
- Identity:HGNC:34918
- Gene:TRH-GTG2-1
- Long gene name:tRNA-His (GTG) 2-1
- Synonyms gene name:transfer RNA histidine 11 (anticodon GUG), transfer RNA-His (GTG) 2-1
- Synonyms:tRNA-His-GTG-2-1,
- Discovery year:2008-08-29
- Entrez gene record:100189350
- Classification:Low confidence cytoplasmic transfer RNAs
- Identity:HGNC:34805
- Gene:TRH-GTG3-1
- Long gene name:tRNA-His (anticodon GTG) 3-1
- Synonyms gene name:transfer RNA histidine 8 (anticodon GUG), transfer RNA-His (GTG) 3-1
- Synonyms:tRNA-His-GTG-3-1,
- Discovery year:2008-08-29
- Entrez gene record:100189242
- Identity:HGNC:34902
- Gene:TRH-GTG1-2
- Long gene name:tRNA-His (anticodon GTG) 1-2
- Synonyms gene name:transfer RNA histidine 10 (anticodon GUG), transfer RNA-His (GTG) 1-2
- Synonyms:tRNA-His-GTG-1-2,
- Discovery year:2008-08-29
- Entrez gene record:100189334
- Classification:Cytoplasmic transfer RNAs
- Identity:HGNC:34736
- Gene:TRH-GTG1-4
- Long gene name:tRNA-His (anticodon GTG) 1-4
- Synonyms gene name:transfer RNA histidine 7 (anticodon GUG), transfer RNA-His (GTG) 1-4
- Synonyms:tRNA-His-GTG-1-4,
- Discovery year:2008-08-29
- Entrez gene record:100189174
- Classification:Cytoplasmic transfer RNAs
- Identity:HGNC:34538
- Gene:TRH-GTG1-5
- Long gene name:tRNA-His (anticodon GTG) 1-5
- Synonyms gene name:transfer RNA histidine 3 (anticodon GUG), transfer RNA-His (GTG) 1-8, transfer RNA-His (GTG) 1-5
- Synonyms:tRNA-His-GTG-1-5,
- Discovery year:2008-09-03
- Entrez gene record:100188987
- Classification:Cytoplasmic transfer RNAs
- Gene symbolHAL, VIS1, AKR1B10, TRH-GTG1-1, TRH-GTG1-3, TRH-GTG2-1, TRH-GTG3-1, TRH-GTG1-2, TRH-GTG1-4, TRH-GTG1-5
- isotype filter
- Immunoglobulin G IgG 1
- LabelHis
- label filter
- His
- technique filter
- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
- Alternative techniqueantibodies