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Smad2(Ser465 + Ser467) Antibody, FITC Conjugated

  • Catalog number
    bs-3419R-FITC
  • Product name
    Smad2(Ser465 + Ser467) Antibody, FITC Conjugated
  • Size
    0.1ml
  • Short name
    Anti-Smad2 Ser465 + Ser467
  • Long name
    Smad2(Ser465 + Ser467) Antibody, FITC Conjugated
  • Category
    Conjugated Primary Antibodies
  • Conjugation
    FITC
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    Smad2 Ser465 + Ser467
  • Specificity
    This is a highly specific antibody against Smad2 Ser465 + Ser467.
  • Modification
    Phosphorylation
  • Modification site
    Ser465 + Ser467
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Source
    KLH conjugated synthetic phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467
  • Gene ID number
    4087
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Crossreactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Antigen background
    Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF alpha receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.
  • Purification method
    This antibody was purified via Protein A.
  • Storage conditions
    Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
  • Excitation Emission
    494nm/518nm
  • Synonyms
    Smad2p-Ser465/467; p-Smad2Ser465/467; Smad2p-S465/467; hMAD 2; hSMAD2; JV18 1; JV18; JV181; MAD; MAD Related Protein 2; MADH2; MADR2; MGC22139; MGC34440; Mothers Against Decapentaplegic Homolog 2; mothers against DPP homolog 2; SMAD 2; SMAD; SMAD2; SMAD2_HUMAN.
  • Alternative to gene target
    • [ "SMAD family member 2", "hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2", "SMAD2 and IDBG-3127 and ENSG00000175387 and 4087", "transforming growth factor beta receptor", "nuclei", "Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126", "SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125,516010" ]
  • Gene target
    Smad2 Ser465 + Ser467 Conjugated
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    FITC
  • label filter
    • FITC
  • technique filter
    • Antibody
    • FITC
  • Technique
    Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
  • Alternative technique
    antibodies, fluorescine