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DNA PKcs ANTIBODY, Cy5 Conjugated

  • Catalog number
    bs-10994R-Cy5
  • Product name
    DNA PKcs ANTIBODY, Cy5 Conjugated
  • Size
    0.1ml
  • Short name
    Anti-DNA PKcs
  • Long name
    DNA PKcs POLYCLONAL ANTIBODY, Cy5 Conjugated
  • Category
    Conjugated Primary Antibodies
  • Conjugation
    Cy5
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    DNA PKcs
  • Specificity
    This is a highly specific antibody against DNA PKcs.
  • Modification
    Unmodified
  • Modification site
    None
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Source
    KLH conjugated synthetic peptide derived from human DNA PKcs
  • Gene ID number
    5591
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Crossreactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Antigen background
    Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate TP53/p53 in the presence of supercoiled DNA is dependent on C1D.
  • Purification method
    This antibody was purified via Protein A.
  • Storage conditions
    Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
  • Excitation Emission
    625,650nm/670nm
  • Synonyms
    DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460; PRKDC; HYRC; HYRC1; Variant; DNAPK; DNAPK catalytic subunit; p350; PRKDC; Protein Kinase DNA Activated Catalytic Polypeptide; XRCC 7; XRCC7; PRKDC_HUMAN.
  • Gene target
    DNA PKcs Conjugated
    • Gene info
    Locus Specific Databases:LRG_162
    Gene info
    Locus Specific Databases:IMGT/HLA Database
  • Gene symbol
    PRKDC, HLA-DOA
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    Cy5
  • label filter
    • Cy5
  • technique filter
    • Antibody
    • dna
  • Technique
    Antibody, dna, antibodies against human proteins, antibodies for, antibody Conjugates
  • Alternative technique
    antibodies