Collagen VII Antibody, FITC Conjugated

• Catalog number
  bs-1558R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  Collagen VII Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  Collagen VII
• Specificity
  This is a highly specific antibody against Collagen VII.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  80-130/2944
• Source
  KLH conjugated synthetic peptide derived from human Collagen VII
• Gene ID number
  1294
• Swiss Prot
  Q02388
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  EBD1; EBR1; EBDCT; Collagen alpha-1(VII) chain; Long-chain collagen; LC collagen; COL7A1
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Gene
  Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  Collagen   VII   Conjugated  
• Gene symbol
  MARCHF7, IGLVVII-41-1, SYT7
• Short name
  Anti-Collagen VII
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-Collagen VII PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:17393
• Gene
  MARCHF7
• Long gene name
  membrane associated ring-CH-type finger 7
• Synonyms gene
  • AXOT
  • MARCH7
• Synonyms gene name
  • axotrophin
  • membrane-associated ring finger (C3HC4) 7
  • membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase
• Synonyms
  • MARCH-VII
  • RNF177
• GenBank acession
  • AK022973
• Locus
  2q24.2
• Discovery year
  2001-12-17
• Entrez gene record
  64844
• Pubmed identfication
  • 14722266
• RefSeq identity
  • NM_022826
• Classification
  • Ring finger proteins
  • Membrane associated ring-CH-type fingers
• VEGA ID
  OTTHUMG00000132029

Gene info

• Identity
  HGNC:15691
• Gene
  IGLVVII-41-1
• Long gene name
  immunoglobulin lambda variable (VII)-41-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable (VII)-41-1
  • immunoglobulin lambda variable (VII)-41-1 pseudogene
• Synonyms
  • IGLV(VII)-41-1
• GenBank acession
  • X99568
• Locus
  22q11.22
• Discovery year
  2001-05-15
• Entrez gene record
  84089
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151296

Gene info

• Identity
  HGNC:11514
• Gene
  SYT7
• Long gene name
  synaptotagmin 7
• Synonyms gene
  • PCANAP7
• Synonyms gene name
  • prostate cancer associated protein 7
  • synaptotagmin VII
• Synonyms
  • IPCA-7
  • SYT-VII
  • MGC150517
• GenBank acession
  • AF038535
• Locus
  11q12.2
• Discovery year
  1999-07-14
• Entrez gene record
  9066
• Pubmed identfication
  • 9615227
• RefSeq identity
  • NM_004200
• Classification
  • Synaptotagmins
• VEGA ID
  OTTHUMG00000168199

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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