- Catalog numberbs-12578R-Biotin
- Product nameBcl2 (Thr69) Antibody, Biotin Conjugated
- Size0.1ml
- PriceAsk For Price
- Short nameAnti-Bcl2 Thr69
- Long nameBcl2 (Thr69) Polyclonal Antibody, Biotin Conjugated
- CategoryConjugated Primary Antibodies
- ConjugationBiotin
- Host OrganismRabbit (Oryctolagus cuniculus)
- Target AntigenBcl2 Thr69
- SpecificityThis is a highly specific antibody against Bcl2 Thr69.
- ModificationPhosphorylation
- Modification siteThr69
- ClonalityPolyclonal
- ClonePolyclonal antibody
- IsotypeImmunoglobulin G (IgG)
- Concentration1ug per 1ul
- SourceKLH conjugated synthetic phosphopeptide derived from human Bcl-2 around the phosphorylation site of Thr69
- Gene ID number596
- Tested applicationsWB, IHC-P
- Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500)
- CrossreactivityHuman
- Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
- Antigen backgroundBCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL
- Purification methodThis antibody was purified via Protein A.
- Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
- Excitation EmissionN/A
- SynonymsBcl2 Thr69; Bcl2 T69; Bcl2 phospho T69; p-Bcl2 phospho T69; Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN; Apoptosis regulator Bcl-2.
- Alternative to gene target
- [ "B-cell CLL/lymphoma 2", "Bcl-2 and PPP1R50", "BCL2 and IDBG-4354 and ENSG00000171791 and 596", "protein phosphatase 2A binding", "nuclei", "Bcl2 and IDBG-185673 and ENSMUSG00000057329 and 12043", "BCL2 and IDBG-645006 and ENSBTAG00000019302 and 281020" ]
- Gene targetBcl2 Thr69 Conjugated
- isotype filter
- Immunoglobulin G IgG
- LabelBiotin
- label filter
- Biotin
- technique filter
- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
- Alternative techniqueantibodies