- Catalog numberbs-2445R-Cy3
- Product nameAurora A+B+C Antibody, Cy3 Conjugated
- Size0.1ml
- PriceAsk For Price
- Short nameAnti-Aurora A+B+C
- Long nameAurora A+B+C Polyclonal Antibody, Cy3 Conjugated
- CategoryConjugated Primary Antibodies
- ConjugationCy3
- Host OrganismRabbit (Oryctolagus cuniculus)
- Target AntigenAurora A+B+C
- SpecificityThis is a highly specific antibody against Aurora A+B+C.
- ModificationUnmodified
- Modification siteNone
- ClonalityPolyclonal
- ClonePolyclonal antibody
- IsotypeImmunoglobulin G (IgG)
- Concentration1ug per 1ul
- Immunogen range70-105/344
- Subcellular locationCytoplasm
- SourceKLH conjugated synthetic peptide derived from human Aurora B
- Gene ID number9212
- Tested applicationsIF(IHC-P)
- Recommended dilutionsIF(IHC-P)(1:50-200)
- CrossreactivityHuman, Mouse
- Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
- Antigen backgroundSerine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes.
- Purification methodThis antibody was purified via Protein A.
- Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
- Excitation Emission512,550nm/570,615nm
- SynonymsAurora 2; Aurora/IPL1-related kinase 1; ARK-1, Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B; Aurora kinase C, Aurora 3, Aurora/IPL1-related kinase 3, ARK-3, Aurora-related kinase 3, Aurora/IPL1/Eg2 protein 2, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase aurora-C
- Gene targetAurora A+B+C Conjugated
- isotype filter
- Immunoglobulin G IgG
- LabelCy3
- label filter
- Cy3
- technique filter
- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
- Alternative techniqueantibodies