- Catalog numberbs-12540R-Cy7
- Product nameATF2(Thr71) Antibody, Cy7 Conjugated
- Size0.1ml
- PriceAsk For Price
- Short nameAnti-ATF2 Thr71
- Long nameATF2(Thr71) Polyclonal Antibody, Cy7 Conjugated
- CategoryConjugated Primary Antibodies
- ConjugationCy7
- Host OrganismRabbit (Oryctolagus cuniculus)
- Target AntigenATF2 Thr71
- SpecificityThis is a highly specific antibody against ATF2 Thr71.
- ModificationPhosphorylation
- Modification siteThr71
- ClonalityPolyclonal
- ClonePolyclonal antibody
- IsotypeImmunoglobulin G (IgG)
- Concentration1ug per 1ul
- SourceKLH conjugated synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Thr71
- Gene ID number1386
- Tested applicationsIF(IHC-P)
- Recommended dilutionsIF(IHC-P)(1:50-200)
- CrossreactivityHuman
- Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
- Antigen backgroundATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
- Purification methodThis antibody was purified via Protein A.
- Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
- Excitation Emission743nm/767nm
- SynonymsATF2 phospho T71; p-ATF2 phospho T71; ATF2 phospho Thr71; p-ATF2 phospho Thr71; CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN.
- Gene targetATF2 Thr71 Conjugated
- Identity:HGNC:784
- Gene:ATF2
- Long gene name:activating transcription factor 2
- Synonyms gene name:cAMP responsive element binding protein 2
- Synonyms:TREB7CRE-BP1HB16
- Discovery year:1991-08-01
- Entrez gene record:1386
- Pubmed identification:1833307, 1838349
- RefSeq identity:NM_001880
- Classification:Basic leucine zipper proteins, MicroRNA protein coding host genes
- VEGA ID:OTTHUMG00000132424
- Gene symbolGDNF, ATF2
- isotype filter
- Immunoglobulin G IgG
- LabelCy7
- label filter
- Cy7
- technique filter
- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
- Alternative techniqueantibodies