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ATF2 (Thr55) Antibody, Cy5.5 Conjugated

  • Catalog number
    bs-12539R-Cy5.5
  • Product name
    ATF2 (Thr55) Antibody, Cy5.5 Conjugated
  • Size
    0.1ml
  • Short name
    Anti-ATF2 Thr55
  • Long name
    ATF2 (Thr55) Polyclonal Antibody, Cy5.5 Conjugated
  • Category
    Conjugated Primary Antibodies
  • Conjugation
    Cy5.5
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    ATF2 Thr55
  • Specificity
    This is a highly specific antibody against ATF2 Thr55.
  • Modification
    Phosphorylation
  • Modification site
    Thr55
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Source
    KLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Thr55
  • Gene ID number
    11909
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Crossreactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Antigen background
    ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
  • Purification method
    This antibody was purified via Protein A.
  • Storage conditions
    Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
  • Excitation Emission
    675nm/694nm
  • Synonyms
    ATF2 phospho T55; p-ATF2 phospho T55; p-ATF2 phospho T73 + T55; CREB 2; HB 16; Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2
  • Gene target
    ATF2 Thr55 Conjugated
    • Gene info
    Gene info
  • Gene symbol
    GDNF, ATF2
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    Cy5.5
  • label filter
    • Cy5.5
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
  • Alternative technique
    antibodies