- Catalog numberbs-13755R-A488
- Product nameZonula occludens protein 3 Antibody, ALEXA FLUOR 488
- Size100ul
- PriceAsk For Price
- Short nameAnti-Zonula occludens protein 3
- Long nameZonula occludens protein 3 Antibody, ALEXA FLUOR 488 Conjugated
- Also known asAnti-Zonula occludens protein 3 PAb ALEXA FLUOR 488
- CategoryConjugated Primary Antibodies
- Conjugated withALEXA FLUOR® 488
- Host OrganismRabbit (Oryctolagus cuniculus)
- Target AntigenZonula occludens protein 3
- SpecificityThis is a highly specific antibody against Zonula occludens protein 3.
- ModificationUnmodified
- Modification SiteNone
- ClonalityPolyclonal
- ClonePolyclonal antibody
- IsotypeImmunoglobulin G (IgG)
- Concentration1ug per 1ul
- SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Zonula occludens protein 3
- Tested applicationsIF(IHC-P)
- Recommended dilutionsIF(IHC-P)(1:50-200)
- CrossreactivityHuman, Mouse, Rat
- Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
- Background of the antigenTight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane-associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3 (1,2). These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain (3). ZO-1 forms complexes with either ZO-2 or ZO-3 (4). In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction (5-7). ZO-1 expression is significantly reduced in many breast cancer lines (8). ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue (2,3).
- PurificationPurified by Protein A.
- Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
- Excitation emission499nm/519nm
- SynonymsTight junction protein 3; Tight junction protein ZO-3; Tight junction protein ZO3; TJP3; ZO3;ZO-3; ZO3_HUMAN; Zona occludens protein 3; Zonula occludens protein 3.
- Gene targetZonula occludens protein 3
- isotype filter
- Immunoglobulin G IgG
- LabelALEXA FLUOR 488
- label filter
- ALEXA FLUOR 488
- technique filter
- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for
- Alternative techniqueantibodies