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IKK beta(Tyr199) Antibody, ALEXA FLUOR 488

  • Catalog number
    bs-3232R-A488
  • Product name
    IKK beta(Tyr199) Antibody, ALEXA FLUOR 488
  • Size
    100ul
  • Short name
    Anti-IKK beta Tyr199
  • Long name
    IKK beta(Tyr199) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
  • Also known as
    Anti-IKK beta Tyr199 PAb ALEXA FLUOR 488
  • Category
    Conjugated Primary Antibodies
  • Conjugated with
    ALEXA FLUOR® 488
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    IKK beta Tyr199
  • Specificity
    This is a highly specific antibody against IKK beta Tyr199.
  • Modification
    Phosphorylation
  • Modification Site
    Tyr199
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Immunogen range
    180-230/756
  • Subcellular location
    Cytoplasm, Nucleus
  • Source
    KLH conjugated synthetic phosphopeptide derived from human IKK beta around the phosphorylation site of Tyr199
  • Gene ID Number
    3551
  • Swiss Prot
    O14920
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Cross reactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Background of the antigen
    Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.
  • Purification
    Purified by Protein A.
  • Storage conditions
    Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
  • Excitation emission
    499nm/519nm
  • Synonyms
    IKK2; IKKB; IMD15; NFKBIKB; IKK-beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IkBKB; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta
  • Gene target
    IKK beta Tyr199
    • Gene info
    Gene info
    Locus Specific Databases:LRG_1241
    Gene info
    Gene info
    Locus Specific Databases:IKBKGbase: Mutation registry for Nemo deficiencyGlobal Variome shared LOVDLRG_70
  • Gene symbol
    IKBKE, IKBKB, CHUK, IKBKG
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    ALEXA FLUOR 488
  • label filter
    • ALEXA FLUOR 488
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Alternative technique
    antibodies