- Catalog numberbs-11645R-A350
- Product nameFISH Antibody, ALEXA FLUOR 350
- Size100ul
- PriceAsk For Price
- Short nameAnti-FISH
- Long nameFISH Polyclonal Antibody, ALEXA FLUOR 350 Conjugated
- Also known asAnti-FISH PAb ALEXA FLUOR 350
- CategoryConjugated Primary Antibodies
- Conjugated withALEXA FLUOR® 350
- Host OrganismRabbit (Oryctolagus cuniculus)
- Target AntigenFISH
- SpecificityThis is a highly specific antibody against FISH.
- ModificationUnmodified
- Modification SiteNone
- ClonalityPolyclonal
- ClonePolyclonal antibody
- IsotypeImmunoglobulin G (IgG)
- Concentration1ug per 1ul
- SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human FISH
- Tested applicationsIF(IHC-P)
- Recommended dilutionsIF(IHC-P)(1:50-200)
- CrossreactivityHuman, Mouse, Rat
- Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
- Background of the antigenFish, a potential Src substrate, is a broadly expressed adaptor protein containing five SH3 domains and a phox homology (PX) domain (1). The Src family of protein tyrosine kinases act in signal transduction pathways (2-4). Src kinases vary in expression but are strongly regulated in vivo; catalytic activity is repressed by interacting with the SH3 domain (5-7). In Src-transformed fibroblasts and in normal cells treated with certain growth factors fish is tyrosine-phosphorylated (1). Treatment of cells with cytochalasin D results in rapid tyrosine phosphorylation of fish, along with activation of Src (1). Fish is likely to be involved in tyrosine kinase signaling and may have a role in cytoskeletal changes (1).
- PurificationPurified by Protein A.
- Storage conditionsStore this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
- Excitation emission343nm/442nm
- SynonymsAdapter protein TKS5; Five SH3 domain-containing protein; SH3 and PX domain-containing protein 2A; SH3 multiple domains protein 1; Sh3md1; Sh3pxd2a; SPD2A_HUMAN; TKs5; Tyrosine kinase substrate with five SH3 domains.
- Gene targetFISH
- Identity:HGNC:23664
- Gene:SH3PXD2A
- Long gene name:SH3 and PX domains 2A
- Synonyms gene name:SH3 multiple domains 1
- Synonyms:FISHKIAA0418
- Discovery year:2003-12-01
- Entrez gene record:9644
- Pubmed identification:9687503
- RefSeq identity:NM_014631
- VEGA ID:OTTHUMG00000018997
- Gene symbolSH3PXD2A
- isotype filter
- Immunoglobulin G IgG
- LabelALEXA FLUOR 350
- label filter
- ALEXA FLUOR 350
- technique filter
- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for
- Alternative techniqueantibodies