DNA Ligase IV/LIG4 Antibody, ALEXA FLUOR 647

• Catalog number
  bs-1677R-A647
• Price
  Please ask
• Size
  100ul

• Long name
  DNA Ligase IV/LIG4 Polyclonal Antibody, ALEXA FLUOR 647 Conjugated
• Also known as
  Anti-DNA Ligase IV/LIG4 PAb ALEXA FLUOR 647
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 647
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  DNA Ligase IV/LIG4
• Specificity
  This is a highly specific antibody against DNA Ligase IV/LIG4.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  60-110/911
• Subcellular location
  Nucleus
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human DNA Ligase IV
• Gene ID Number
  3981
• Swiss Prot
  P49917
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  650nm/665nm
• Synonyms
  LIG4S; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4
• Properties
  For facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-DNA Ligase IV/LIG4 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor, ALEXA FLUOR 647
• French translation
  anticorps

• Gene target
  DNA   Ligase   IV/LIG4  
• Gene symbol
  LIG4, PRKDC, HLA-DOA
• Short name
  Anti-DNA Ligase IV/LIG4
• Technique
  Antibody, dna, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 647
• Alternative name
  DNA Ligase IV/LIG4 Polyclonal Antibody, ALEXA FLUOR 647
• Alternative technique
  antibodies
• Alternative to gene target
  ligase IV, DNA, ATP-dependent, LIG4S, LIG4 and IDBG-49744 and ENSG00000174405 and 3981, metal ion binding, nuclei, Lig4 and IDBG-131943 and ENSMUSG00000049717 and 319583, LIG4 and IDBG-633610 and ENSBTAG00000015868 and 781252

Gene info

• Identity
  HGNC:6601
• Gene
  LIG4
• Long gene name
  DNA ligase 4
• Synonyms gene name
  • ligase IV, DNA, ATP-dependent
• Synonyms name
  • polydeoxyribonucleotide synthase [ATP] 4
  • polynucleotide ligase
  • sealase
  • DNA repair enzyme
  • DNA joinase
  • DNA ligase IV
• GenBank acession
  • X83441
• Locus
  13q33.3
• Discovery year
  1995-08-10
• Entrez gene record
  3981
• Pubmed identfication
  • 7760816
• RefSeq identity
  • NM_002312
• Classification
  • DNA ligases
• VEGA ID
  OTTHUMG00000017328
• Locus Specific Databases
  • LIG4base: Mutation registry for LIG4 syndrome
  • LRG_79

Gene info

• Identity
  HGNC:9413
• Gene
  PRKDC
• Long gene name
  protein kinase, DNA-activated, catalytic subunit
• Synonyms gene
  • HYRC
  • HYRC1
• Synonyms gene name
  • protein kinase, DNA-activated, catalytic polypeptide
• Synonyms
  • DNPK1
  • p350
  • DNAPK
  • XRCC7
  • DNA-PKcs
  • DNAPKc
  • DNA-PKC
  • p460
• Synonyms name
  • DNA-dependent protein kinase catalytic subunit
• Locus
  8q11.21
• Discovery year
  1993-11-09
• Entrez gene record
  5591
• Pubmed identfication
  • 7638222
• RefSeq identity
  • NM_001081640
• Classification
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000164239
• Locus Specific Databases
  • LRG_162

Gene info

• Identity
  HGNC:4936
• Gene
  HLA-DOA
• Long gene name
  major histocompatibility complex, class II, DO alpha
• Synonyms gene
  • HLA-DZA
  • HLA-DNA
• Synonyms
  • HLA-D0-alpha
• GenBank acession
  • M31525
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3111
• Pubmed identfication
  • 2370084
• RefSeq identity
  • NM_002119
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031211
• Locus Specific Databases
  • IMGT/HLA Database

MeSH Data

• Name
  Ligase Chain Reaction
• Concept
  Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
• Tree numbers
  • E05.393.620.311
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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