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Anti-ATF2 (Thr55) (Polyclonal), ALEXA Fluor 594

  • Catalog number
    GENTObs-12539R-A594
  • Product name
    Anti-ATF2 (Thr55) (Polyclonal), ALEXA Fluor 594
  • Size
    100 microliters
  • Short name
    Anti-ATF2 (Thr55)
  • Type
    Conjugated Primary Antibody
  • Conjugated with
    ALEXA FLUOR® 594
  • Host organism
    Rabbit (Oryctolagus cuniculus)
  • Target Protein Peptide
    ATF2 Thr55
  • Specificity
    This antibody reacts specifically with ATF2 (Thr55)
  • Modification
    Phosphorylation
  • Modification site
    Thr55
  • Clonality
    Polyclonal Antibody
  • Clone
    Polyclonal Antibodies
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Subcellular locations
    N/A
  • Antigen Source
    KLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Thr55
  • Gene ID
    11909
  • Swiss Prot
    N/A
  • Applications
    IF(IHC-P)
  • Applications with corresponding dilutions
    IF(IHC-P)(1:50-200)
  • Cross reactive species
    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
  • Cross Reactive Species details
    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
  • Background information
    ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
  • Purification method
    Purified by Protein A.
  • Storage
    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
  • Excitation emission
    590nm/617nm
  • Synonyms
    ATF2 phospho T55; p-ATF2 phospho T55; p-ATF2 phospho T73 + T55; CREB 2; HB 16; Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2
  • Also known as
    ATF2 (Thr55) Polyclonal Antibody
  • Other name
    Anti-ATF2 (Thr55) Polyclonal
  • Advisory
    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
  • Gene target
    ATF2 Thr55
    • Gene info
    Gene info
  • Gene symbol
    GDNF, ATF2
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    ALEXA FLUOR 594
  • label filter
    • ALEXA FLUOR 594
  • technique filter
    • Polyclonal
    • anti-
    • anti
  • Technique
    Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, antibodies