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Anti-A Raf (Tyr301 + Tyr302), ALEXA Fluor 594

  • Catalog number
    GENTObs-3421R-A594
  • Product name
    Anti-A Raf (Tyr301 + Tyr302), ALEXA Fluor 594
  • Size
    100 microliters
  • Short name
    Anti-A Raf (Tyr301 + Tyr302)
  • Type
    Conjugated Primary Antibody
  • Conjugated with
    ALEXA FLUOR® 594
  • Host organism
    Rabbit (Oryctolagus cuniculus)
  • Target Protein Peptide
    A Raf Tyr301 + Tyr302
  • Specificity
    This antibody reacts specifically with A Raf (Tyr301 + Tyr302)
  • Modification
    Phosphorylation
  • Modification site
    Tyr301 + Tyr302
  • Clonality
    Polyclonal Antibody
  • Clone
    Polyclonal Antibodies
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Subcellular locations
    N/A
  • Antigen Source
    KLH conjugated synstic phosphopeptide derived from human A Raf around the phosphorylation site of Tyr301/302 [G(p-Y)(p-Y)WE]
  • Gene ID
    369
  • Swiss Prot
    N/A
  • Applications
    IF(IHC-P)
  • Applications with corresponding dilutions
    IF(IHC-P)(1:50-200)
  • Cross reactive species
    Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
  • Cross Reactive Species details
    No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
  • Background information
    The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
  • Purification method
    Purified by Protein A.
  • Storage
    Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
  • Excitation emission
    590nm/617nm
  • Synonyms
    A RAF phospho Y301 + Y302; p-A RAF phospho Y301 + Y302; A raf 1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 murine sarcoma 3611 virus; ARAF_HUMAN.
  • Also known as
    A Raf (Tyr301 + Tyr302) Antibody
  • Other name
    Anti-A Raf (Tyr301 + Tyr302)
  • Advisory
    Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
  • Gene target
    A Raf Tyr301 + Tyr302
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    ALEXA FLUOR 594
  • label filter
    • ALEXA FLUOR 594
  • technique filter
    • anti-
    • anti
  • Technique
    anti-, anti, antibody to, antibodies
  • Alternative technique
    antibodies