- Catalog numberEKU07441
- Product nameRat Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit
- Size96 Tests
- PriceAsk For Price
- Estimated Turnaround Time6-11 Business days
- Target NameSpleen Focus Forming Virus Proviral Integration Oncogene
- Target s alterntive nameOF, PU.1, SFPI1, SPI-1, SPI-A, SPI-1 Proto-Oncogene, Hematopoietic Transcription Factor PU.1, 31 kDa Transforming Protein
- Species ReactivityRattus norvegicus (Rat)
- Sample TypeTissue homogenates, Cell lysates and other biological fluids.
- Detection Range0.312-20 ng/mL
- Sensitivity0.116 ng/mL
- PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spleen Focus Forming Virus Proviral Integration Oncogene were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spleen Focus Forming Virus Proviral Integration Oncogene were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
- Detection MethodColorimetric
- Assay Time3 hours
- ApplicationFor research use only. Not for diagnostic procedures.
- Assay TypeDouble-antibody Sandwich
- Shipping ConditionsIce packs
- Storage TemperatureShort term: 4°C; Long term: see manual.
- Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
- Shelf lifeUse Rat Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit before 8 months
- SpecificityThis assay has high sensitivity and excellent specificity for detection of Spleen Focus Forming Virus Proviral Integration Oncogene
- Cross ActivityNo significant cross-reactivity or interference between Spleen Focus Forming Virus Proviral Integration Oncogene and analogues was observed.
- Test PrincipleNo significant cross-reactivity or interference between Spleen Focus Forming Virus Proviral Integration Oncogene and analogues was observed.
- StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Quality SystemsThe kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
- Research AreaTumor immunity
- Alternative to gene target
- [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
- [ "spleen focus forming virus (SFFV) proviral integration oncogene spi1", "OF and PU.1 and SFPI1 and SPI-1 and SPI-A", "SPI1 and IDBG-43260 and ENSG00000066336 and 6688", "NFAT protein binding", "nuclei", "Sfpi1 and IDBG-190185 and ENSMUSG00000002111 and 20375", "BT.58934 and IDBG-637435 and ENSBTAG00000021709 and 507100" ]
- Gene targetSpleen Focus Forming Virus Proviral Integration Oncogene SPI1
- Identity:HGNC:11241
- Gene:SPI1
- Long gene name:Spi-1 proto-oncogene
- Synonyms gene name:spleen focus forming virus (SFFV) proviral integration oncogene
- Synonyms:PU.1SPI-AOFSFPI1SPI-1
- Discovery year:1988-07-04
- Entrez gene record:6688
- Pubmed identification:1693183
- RefSeq identity:NM_003120
- Classification:ETS transcription factor family
- VEGA ID:OTTHUMG00000150150
- Gene symbolSPI1
- HostRat, antigen assay ELISA kit 1 Kit (96 Wells)
- Short nameSpleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit
- technique filter
- ELISA kit
- ELISA
- TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
- Alternative techniqueelisas, kits
- Tissuespleen
- tissue filter
- spleen