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Rat PYD (Pyridinoline) ELISA Kit

  • Catalog number
    EKE62258
  • Product name
    Rat PYD (Pyridinoline) ELISA Kit
  • Size
    96 Tests
  • Estimated Turnaround Time
    6-11 business days
  • Target Name
    NA
  • Target s alterntive name
    PYD
  • Species Reactivity
    Rat
  • Sample Type
    Serum, Plasma, Biological Fluids
  • Detection Range
    6.25-400 ng/mL
  • Sensitivity
    3.75 ng/mL
  • Precision
    NA
  • Detection Method
    Colorimetric
  • Assay Time
    2.5 hours
  • Application
    For research use only. Not for diagnostic procedures.
  • Assay Type
    Competitive
  • Shipping Conditions
    Ice packs
  • Storage Temperature
    Short term: 4°C; Long term: see manual.
  • Precaution of Use
    The Stop Solution is acidic. Do not allow to contact skin or eyes.
  • Shelf life
    Use Rat PYD (Pyridinoline) ELISA Kit before 6 months
  • Specificity
    NA
  • Cross Activity
    No significant cross-reactivity or interference was observed.
  • Test Principle
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat PYD. During the reaction, Rat PYD in the sample or standard competes with a fixed amount of Rat PYD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PYD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Rat PYD in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Stability
    NA
  • Quality Systems
    The kit is manufactured at ISO 9001 certified facilities.
  • Research Area
    NA
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    PYD Pyridinoline
  • Host
    Rat, antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    PYD (Pyridinoline) ELISA Kit
  • technique filter
    • ELISA kit
    • ELISA
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Alternative technique
    elisas, kits