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Vitamin D2 (VD2) CLIA Kit, 96 well plate

  • Catalog number
    EKU08649
  • Product name
    Vitamin D2 (VD2) CLIA Kit, 96 well plate
  • Size
    10x96T
  • Verified reactivity
    Multispecies (General)
  • Detection limits
    1.17-300 ng/mL
  • Sensitivity limit
    0,45 ng/mL
  • Samples to be analyzed
    Serum, plasma and other biological fluids
  • Assay duration
    2 hours
  • Verified applications
    CLIA
  • Assay class
    Competitive
  • ELISA detection
    Chemiluminescent
  • Protein number
    Please refer to Uniprot
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    Please refer to GenBank
  • Gene name
    Vitamin D2
  • Alternate gene name
    Ergocalciferol; Deltalin; Drisdol; Calcidol
  • Estimated production time
    7-11 business days
  • Shipping requirements
    Blue ice
  • Storage recommendation
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    8 months
  • ELISA s specificity
    This assay has high sensitivity and excellent specificity for detection of Vitamin D2 (VD2). No significant cross-reactivity or interference between Vitamin D2 (VD2) and analogues was observed.
  • ELISA s cross reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Vitamin D2 (VD2). A competitive inhibition reaction is launched between biotin labeled Vitamin D2 (VD2) and unlabeled Vitamin D2 (VD2) (Standards or samples) with the pre-coated antibody specific to Vitamin D2 (VD2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin D2 (VD2) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Vitamin D2 (VD2) level in the sample or standard.
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D2 (VD2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D2 (VD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA s stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    Metabolic pathway;Neuro science;Nutrition metabolism;Bone metabolism;
  • Warnings
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Hyperlink
    Hyperlink
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Vitamin D2 VD2 CLIA Kit 96
  • Short name
    Vitamin D2 (VD2) CLIA Kit, 96
  • Alternative technique
    kits