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Rat Vascular Endothelial Growth Factor A (VEGFA) CLIA Kit, 96 well plate

  • Catalog number
    EKU08850
  • Product name
    Rat Vascular Endothelial Growth Factor A (VEGFA) CLIA Kit, 96 well plate
  • Size
    10x96T
  • Verified reactivity
    Rattus norvegicus (Rat)
  • Detection limits
    31.25-2000 pg/mL
  • Sensitivity limit
    0,99 pg/mL
  • Samples to be analyzed
    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Assay duration
    2 hours, 40min
  • Verified applications
    CLIA
  • Assay class
    Sandwich
  • ELISA detection
    Chemiluminescent
  • Protein number
    P16612
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    83785
  • Gene name
    Vascular Endothelial Growth Factor A
  • Alternate gene name
    VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
  • Estimated production time
    7-11 business days
  • Shipping requirements
    Blue ice
  • Storage recommendation
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    8 months
  • ELISA s specificity
    This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor A (VEGFA). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.
  • ELISA s cross reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    The microplate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vascular Endothelial Growth Factor A (VEGFA) level in the sample or standard.;
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA s stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    Cytokine;Apoptosis;Tumor immunity;
  • Warnings
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Hyperlink
    Hyperlink
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
    • [ "vascular endothelial growth factor A", "MVCD1 and VEGF and VPF", "VEGFA and IDBG-88716 and ENSG00000112715 and 7422", "extracellular matrix binding", "Extracellular", "Vegfa and IDBG-185847 and ENSMUSG00000023951 and 22339", "VEGFA and IDBG-631944 and ENSBTAG00000047561 and 101904706" ]
  • Gene target
    Vascular Endothelial Growth Factor A VEGFA CLIA Kit 96
    • Gene info
    Locus Specific Databases:ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database
  • Gene symbol
    VEGFA
  • Host
    Rat
  • Short name
    Vascular Endothelial Growth Factor A (VEGFA) CLIA Kit, 96
  • Alternative technique
    kits
  • Tissue
    vascular
  • tissue filter
    • vascular