Rat Procollagen III N-Terminal Propeptide (PIIINP) CLIA Kit, 96 well plate

• Catalog number
  EKU09956
• Price
  Please ask
• Size
  10x96T

• Verified reactivity
  Rattus norvegicus (Rat)
• Detection limits
  1.25-80 ng/mL
• Sensitivity limit
  0,05 ng/mL
• Samples to be analyzed
  Serum, plasma and other biological fluids
• Assay duration
  2 hours, 40min
• Verified applications
  CLIA
• Assay class
  Sandwich
• ELISA detection
  Chemiluminescent
• Protein number
  Please refer to Uniprot
• Alternate protein number
  Please refer to SwissProt
• Gene number
  Please refer to GenBank
• Gene name
  Procollagen III N-Terminal Propeptide
• Alternate gene name
  P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide
• Estimated production time
  7-11 business days
• Shipping requirements
  Blue ice
• Storage recommendation
  -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
• Use for
  8 months
• ELISA s specificity
  This assay has high sensitivity and excellent specificity for detection of Procollagen III N-Terminal Propeptide (PIIINP). No significant cross-reactivity or interference between Procollagen III N-Terminal Propeptide (PIIINP) and analogues was observed.
• ELISA s cross reactivity
  This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
• Assay principle
  The microplate provided in this kit has been pre-coated with an antibody specific to Procollagen III N-Terminal Propeptide (PIIINP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Procollagen III N-Terminal Propeptide (PIIINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Procollagen III N-Terminal Propeptide (PIIINP) level in the sample or standard.;
• Protocol
  Please see ELISA's datasheet, otherwise contact us
• Precision of the test
  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen III N-Terminal Propeptide (PIIINP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen III N-Terminal Propeptide (PIIINP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
• ELISA s stability
  The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
• QC
  The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
• Research main area
  Metabolic pathway;Hepatology;Bone metabolism;
• Warnings
  Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
• Notes
  For research use only. Not for diagnostic procedures.
• Hyperlink
• Description
  A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  Procollagen   III   Propeptide   PIIINP   CLIA   Kit  
• Gene symbol
  CPLX3, MGAT3, MGST3, P4HA3, CASP5, THAP4, IGHVIII-82, MT-CSB3, IGHVIII-67-4, IGHVIII-76-1
• Short name
  Procollagen III N-Terminal Propeptide (PIIINP) CLIA Kit, 96
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  Rat Procollagen III N-Terminal Propeptide (PIIINP) CLIA reagent, 96 well plate
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:27652
• Gene
  CPLX3
• Long gene name
  complexin 3
• Synonyms
  • CPX-III
• GenBank acession
  • BC018026
• Locus
  15q24.1
• Discovery year
  2005-08-02
• Entrez gene record
  594855
• Pubmed identfication
  • 15911881
• RefSeq identity
  • NM_001030005
• VEGA ID
  OTTHUMG00000142816

Gene info

• Identity
  HGNC:7046
• Gene
  MGAT3
• Long gene name
  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• Synonyms gene name
  • mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• Synonyms
  • GNT-III
• GenBank acession
  • D13789
• Locus
  22q13.1
• Discovery year
  1993-08-27
• Entrez gene record
  4248
• Pubmed identfication
  • 8370666
• RefSeq identity
  • NM_002409
• Classification
  • Mannosyl-glycoprotein N-acetylglucosaminyltransferases
• VEGA ID
  OTTHUMG00000030185

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:30135
• Gene
  P4HA3
• Long gene name
  prolyl 4-hydroxylase subunit alpha 3
• Synonyms gene name
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III
  • prolyl 4-hydroxylase, alpha polypeptide III
• Synonyms
  • C-P4Halpha(III)
• Synonyms name
  • collagen prolyl 4-hydroxylase alpha(III)
• GenBank acession
  • AY327887
• Locus
  11q13.4
• Discovery year
  2004-05-12
• Entrez gene record
  283208
• Pubmed identfication
  • 14500733
• RefSeq identity
  • NM_182904
• VEGA ID
  OTTHUMG00000165254

Gene info

• Identity
  HGNC:1506
• Gene
  CASP5
• Long gene name
  caspase 5
• Synonyms gene name
  • caspase 5, apoptosis-related cysteine protease
  • caspase 5, apoptosis-related cysteine peptidase
• Synonyms
  • ICE(rel)III
• Locus
  11q22.3
• Discovery year
  1996-09-13
• Entrez gene record
  838
• Pubmed identfication
  • 7797592
  • 9250871
• RefSeq identity
  • NM_004347
• Classification
  • Caspases
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000048073

Gene info

• Identity
  HGNC:23187
• Gene
  THAP4
• Long gene name
  THAP domain containing 4
• Synonyms
  • CGI-36
  • Nb(III)
• Synonyms name
  • nitrobindin
• GenBank acession
  • AF258556
• Locus
  2q37.3
• Discovery year
  2003-10-08
• Entrez gene record
  51078
• Pubmed identfication
  • 12575992
  • 10810093
  • 30524950
• RefSeq identity
  • NM_015963
• Classification
  • THAP domain containing
• VEGA ID
  OTTHUMG00000133410

Gene info

• Identity
  HGNC:5709
• Gene
  IGHVIII-82
• Long gene name
  immunoglobulin heavy variable (III)-82 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-82
  • immunoglobulin heavy variable (III)-82 pseudogene
• Synonyms
  • IGHV(III)-82
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28338
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151732

Gene info

• Identity
  HGNC:7426
• Gene
  MT-CSB3
• Long gene name
  mitochondrially encoded conserved sequence block III
• Synonyms gene
  • MTCSB3
• Synonyms gene name
  • conserved sequence block III
• Synonyms
  • CSB-III
• Locus
  mitochondria
• Discovery year
  1989-10-11
• Pubmed identfication
  • 2982153
• Classification
  • Mitochondrially encoded regions

Gene info

• Identity
  HGNC:5707
• Gene
  IGHVIII-67-4
• Long gene name
  immunoglobulin heavy variable (III)-67-4 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-67-4
  • immunoglobulin heavy variable (III)-67-4 pseudogene
• Synonyms
  • IGHV(III)-67-4
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28340
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151874

Gene info

• Identity
  HGNC:5708
• Gene
  IGHVIII-76-1
• Long gene name
  immunoglobulin heavy variable (III)-76-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-76-1
  • immunoglobulin heavy variable (III)-76-1 pseudogene
• Synonyms
  • IGHV(III)-76-1
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28339
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151872

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