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Human Testosterone - Total ELISA Kit, 96 well plate

  • Catalog number
    EKD01031
  • Product name
    Human Testosterone - Total ELISA Kit, 96 well plate
  • Size
    96T
  • Verified reactivity
    Homo sapiens (Human)
  • Detection limits
    0-16.7 ng/ml
  • Sensitivity limit
    0,022 ng/ml
  • Samples to be analyzed
    Human Serum
  • Assay duration
    2 hours
  • Verified applications
    ELISA
  • Assay class
    Competitive
  • ELISA detection
    Colorimetric
  • Protein number
    Please refer to Uniprot
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    Please refer to GenBank
  • Gene name
    Please refer to GenBank
  • Alternate gene name
    Please refer to NCBI
  • Estimated production time
    4-7 business days
  • Shipping requirements
    Ambient shipping
  • Storage recommendation
    4°C. Bring all reagents to room temperature before beginning test. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    1 year
  • ELISA s specificity
    To know the specificity of this test please feel free to contact us
  • ELISA s cross reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of testosterone in the sample. A set of standards is used to plot a standard curve from which the amount of testosterone in patient samples and controls can be directly read.
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    The intra assay precision is between a CV% of 7.2-11.6 ng/ml. The inter assay precision is between a CV% of 6.1-8.5 ng/ml.
  • ELISA s stability
    Please see ELISA's datasheet, otherwise contact us
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    Biology
  • Warnings
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Hyperlink
    Hyperlink
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Testosterone - 96
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    Testosterone - ELISA Kit, 96
  • technique filter
    • ELISA kit
    • ELISA
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Alternative technique
    elisas, kits
  • Tissue
    total
  • tissue filter
    • total