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Human Spleen Tyrosine Kinase (SYK) ELISA Kit, 96 well plate

  • Catalog number
    EKU07442
  • Product name
    Human Spleen Tyrosine Kinase (SYK) ELISA Kit, 96 well plate
  • Size
    96T
  • Verified reactivity
    Homo sapiens (Human)
  • Detection limits
    0.312-20 ng/mL
  • Sensitivity limit
    0,118 ng/mL
  • Samples to be analyzed
    Tissue homogenates, cell lysates and other biological fluids
  • Assay duration
    3 hours
  • Verified applications
    ELISA
  • Assay class
    Sandwich
  • ELISA detection
    Colorimetric
  • Protein number
    P43405
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    6850
  • Gene name
    Spleen Tyrosine Kinase
  • Alternate gene name
    p72-Syk; Spleen tyrosine kinase
  • Estimated production time
    7-11 business days
  • Shipping requirements
    Blue ice
  • Storage recommendation
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    8 months
  • ELISA s specificity
    This assay has high sensitivity and excellent specificity for detection of Spleen Tyrosine Kinase (SYK). No significant cross-reactivity or interference between Spleen Tyrosine Kinase (SYK) and analogues was observed.
  • ELISA s cross reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spleen Tyrosine Kinase (SYK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spleen Tyrosine Kinase (SYK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spleen Tyrosine Kinase (SYK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spleen Tyrosine Kinase (SYK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spleen Tyrosine Kinase (SYK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spleen Tyrosine Kinase (SYK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA s stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    Enzyme & Kinase;
  • Warnings
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Hyperlink
    Hyperlink
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
    • [ "spleen tyrosine kinase", "p72-Syk", "SYK and IDBG-75571 and ENSG00000165025 and 6850", "transferase activity", "Plasma membranes", "Syk and IDBG-152695 and ENSMUSG00000021457 and 20963", "SYK and IDBG-637026 and ENSBTAG00000004767 and 515515" ]
  • Gene target
    Spleen Tyrosine Kinase SYK 96
    • Gene info
  • Gene symbol
    SYK
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Short name
    Spleen Tyrosine Kinase (SYK) ELISA Kit, 96
  • technique filter
    • ELISA kit
    • ELISA
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Alternative technique
    elisas, kits
  • Tissue
    spleen
  • tissue filter
    • spleen