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Human RAB5A, Member RAS Oncogene Family (RAB5A) CLIA Kit, 96 well plate

  • Catalog number
    EKU09908
  • Product name
    Human RAB5A, Member RAS Oncogene Family (RAB5A) CLIA Kit, 96 well plate
  • Size
    96T
  • Verified reactivity
    Homo sapiens (Human)
  • Detection limits
    0.594-38 ng/mL
  • Sensitivity limit
    0,02 ng/mL
  • Samples to be analyzed
    Tissue homogenates and other biological fluids
  • Assay duration
    2 hours, 40min
  • Verified applications
    CLIA
  • Assay class
    Sandwich
  • ELISA detection
    Chemiluminescent
  • Protein number
    P20339
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    5868
  • Gene name
    RAB5A, Member RAS Oncogene Family
  • Alternate gene name
    RAB5
  • Estimated production time
    7-11 business days
  • Shipping requirements
    Blue ice
  • Storage recommendation
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    8 months
  • ELISA s specificity
    This assay has high sensitivity and excellent specificity for detection of RAB5A, Member RAS Oncogene Family (RAB5A). No significant cross-reactivity or interference between RAB5A, Member RAS Oncogene Family (RAB5A) and analogues was observed.
  • ELISA s cross reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    The microplate provided in this kit has been pre-coated with an antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the RAB5A, Member RAS Oncogene Family (RAB5A) level in the sample or standard.;
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA s stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    Tumor immunity;
  • Warnings
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Hyperlink
    Hyperlink
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
    • [ "RAB5A, member RAS oncogene family", "RAB5", "RAB5A and IDBG-21702 and ENSG00000144566 and 5868", "GDP-dissociation inhibitor binding", "nuclei", "Rab5a and IDBG-190768 and ENSMUSG00000017831 and 271457", "BT.24043 and IDBG-631304 and ENSBTAG00000046385 and 539764" ]
  • Gene target
    RAB5A Member RAS Oncogene Family RAB5A CLIA Kit 96
    • Gene info
    Gene info
    Locus Specific Databases:NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IVLRG_92
    Gene info
    Gene info
    • Identity:HGNC:9876
    • Gene:RASGRF2
    • Long gene name:Ras protein specific guanine nucleotide releasing factor 2
    • Synonyms:GRF2Ras-GRF2
    • Discovery year:1996-12-20
    • Entrez gene record:5924
    • RefSeq identity:NM_006909
    • Classification:Pleckstrin homology domain containing, Dbl family Rho GEFs
    • VEGA ID:OTTHUMG00000119015
    Gene info
  • Gene symbol
    RAB5A, NRAS, RRAS, RASGRF2, MRAS
  • Short name
    RAB5A, Member RAS Oncogene Family (RAB5A) CLIA Kit, 96
  • Alternative technique
    kits