gentaur logogentaur logo
x

Human Intercellular Adhesion Molecule 1 (ICAM1) CLIA Kit, 96 well plate

  • Catalog number
    EKU08885
  • Product name
    Human Intercellular Adhesion Molecule 1 (ICAM1) CLIA Kit, 96 well plate
  • Size
    10x96T
  • Verified reactivity
    Homo sapiens (Human)
  • Detection limits
    39.06-10000 pg/mL
  • Sensitivity limit
    4,8 pg/mL
  • Samples to be analyzed
    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Assay duration
    2 hours, 40min
  • Verified applications
    CLIA
  • Assay class
    Sandwich
  • ELISA detection
    Chemiluminescent
  • Protein number
    P05362
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    3383
  • Gene name
    Intercellular Adhesion Molecule 1
  • Alternate gene name
    CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor
  • Estimated production time
    7-11 business days
  • Shipping requirements
    Blue ice
  • Storage recommendation
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    8 months
  • ELISA s specificity
    This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 1 (ICAM1). No significant cross-reactivity or interference between Intercellular Adhesion Molecule 1 (ICAM1) and analogues was observed.
  • ELISA s cross reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    The microplate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Intercellular Adhesion Molecule 1 (ICAM1) level in the sample or standard.;
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA s stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    CD & Adhesion molecule;Tumor immunity;Infection immunity;
  • Warnings
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Hyperlink
    Hyperlink
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
    • [ "intercellular adhesion molecule 1", "BB2 and CD54 and P3.58", "ICAM1 and IDBG-26804 and ENSG00000090339 and 3383", "protein complex binding", "Extracellular", "Icam1 and IDBG-138668 and ENSMUSG00000037405 and 15894", "ICAM1 and IDBG-643247 and ENSBTAG00000010303 and 281839" ]
  • Gene target
    Intercellular Adhesion Molecule 1 ICAM1 CLIA Kit 96
    • Gene info
    • Identity:HGNC:5344
    • Gene:ICAM1
    • Long gene name:intercellular adhesion molecule 1
    • Synonyms:BB2CD54
    • Discovery year:1989-04-24
    • Entrez gene record:3383
    • Pubmed identification:2453850, 3871395
    • Classification:Ig-like cell adhesion molecule family, Immunoglobulin like domain containing, Receptor ligands, CD molecules
    • VEGA ID:OTTHUMG00000180403
  • Gene symbol
    ICAM1
  • Short name
    Intercellular Adhesion Molecule 1 (ICAM1) CLIA Kit, 96
  • Alternative technique
    kits