- Catalog numberEKU09838
- Product nameHuman Estrogen Receptor Beta (ERb) CLIA Kit, 96 well plate
- Size10x96T
- PriceAsk For Price
- Verified reactivityHomo sapiens (Human)
- Detection limits312.50-20000 pg/mL
- Sensitivity limit10,17 pg/mL
- Samples to be analyzedTissue homogenates and other biological fluids
- Assay duration2 hours, 40min
- Verified applicationsCLIA
- Assay classSandwich
- ELISA detectionChemiluminescent
- Protein numberQ92731
- Alternate protein numberPlease refer to SwissProt
- Gene number2100
- Gene nameEstrogen Receptor Beta
- Alternate gene nameER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3,Group A,Member 2
- Estimated production time7-11 business days
- Shipping requirementsBlue ice
- Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Use for8 months
- ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb). No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.
- ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
- Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Estrogen Receptor Beta (ERb) level in the sample or standard.;
- ProtocolPlease see ELISA's datasheet, otherwise contact us
- Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
- ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
- Research main areaEndocrinology;Reproductive science;Hormone metabolism;
- WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- NotesFor research use only. Not for diagnostic procedures.
- Hyperlink
- Alternative to gene target
- [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
- Gene targetEstrogen Receptor Beta ERb CLIA Kit 96
- Short nameEstrogen Receptor Beta (ERb) CLIA Kit, 96
- Alternative techniquekits