- Catalog numberEKU10043
- Product nameHuman Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) CLIA Kit, 96 well plate
- Size10x96T
- PriceAsk For Price
- Verified reactivityHomo sapiens (Human)
- Detection limits370.37-30000 pg/mL
- Sensitivity limit16,21 pg/mL
- Samples to be analyzedSerum, plasma and other biological fluids
- Assay duration2 hours, 40min
- Verified applicationsCLIA
- Assay classSandwich
- ELISA detectionChemiluminescent
- Protein numberP08727
- Alternate protein numberPlease refer to SwissProt
- Gene number3880
- Gene nameCytokeratin Fragment Antigen 21-1
- Alternate gene namePlease refer to NCBI
- Estimated production time7-11 business days
- Shipping requirementsBlue ice
- Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Use for8 months
- ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Cytokeratin Fragment Antigen 21-1 (CYFRA21-1). No significant cross-reactivity or interference between Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) and analogues was observed.
- ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
- Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) level in the sample or standard.;
- ProtocolPlease see ELISA's datasheet, otherwise contact us
- Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
- ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
- Research main areaBiology
- WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- NotesFor research use only. Not for diagnostic procedures.
- Hyperlink
- Alternative to gene target
- [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
- Gene targetCytokeratin 21-1 CYFRA21-1 CLIA Kit 96
- Identity:HGNC:34943
- Gene:TRC-GCA21-1
- Long gene name:tRNA-Cys (anticodon GCA) 21-1
- Synonyms gene name:transfer RNA cysteine 25 (anticodon GCA), transfer RNA-Cys (GCA) 21-1
- Synonyms:tRNA-Cys-GCA-21-1,
- Discovery year:2008-08-29
- Entrez gene record:100189375
- Classification:Cytoplasmic transfer RNAs
- Identity:HGNC:34897
- Gene:TRSUP-TTA2-1
- Long gene name:tRNA suppressor (anticodon TTA) 2-1
- Synonyms gene name:transfer RNA suppressor 2 (anticodon UUA), transfer RNA suppressor (TTA) 2-1
- Synonyms:tRNA-Sup-TTA-2-1,
- Discovery year:2008-08-29
- Entrez gene record:100189330
- Identity:HGNC:34755
- Gene:TRA-AGC21-1
- Long gene name:tRNA-Ala (AGC) 21-1
- Synonyms gene name:transfer RNA alanine 21 (anticodon AGC), transfer RNA-Ala (AGC) 21-1
- Synonyms:tRNA-Ala-AGC-21-1,
- Discovery year:2008-08-29
- Entrez gene record:100189192
- Classification:Low confidence cytoplasmic transfer RNAs
- Identity:HGNC:38584
- Gene:TRN-GTT21-1
- Long gene name:tRNA-Asn (anticodon GTT) 21-1
- Synonyms gene name:transfer RNA asparagine 38 (anticodon GUU), transfer RNA-Asn (GTT) 21-1
- Synonyms:tRNA-Asn-GTT-21-1,
- Discovery year:2010-06-23
- Entrez gene record:100873961
- Classification:Cytoplasmic transfer RNA pseudogenes
- Identity:HGNC:34692
- Gene:TRL-CAG2-1
- Long gene name:tRNA-Leu (anticodon CAG) 2-1
- Synonyms gene name:transfer RNA leucine 13 (anticodon CAG), transfer RNA-Leu (CAG) 2-1
- Synonyms:tRNA-Leu-CAG-2-1,
- Discovery year:2008-08-29
- Entrez gene record:100189130
- Classification:Cytoplasmic transfer RNAs
- Identity:HGNC:5670
- Gene:IGHVII-1-1
- Long gene name:immunoglobulin heavy variable (II)-1-1 (pseudogene)
- Synonyms gene name:immunoglobulin heavy variable (II)-1-1, immunoglobulin heavy variable (II)-1-1 pseudogene
- Synonyms:IGHV(II)-1-1,
- Discovery year:2000-04-17
- Entrez gene record:28377
- RefSeq identity:NG_001019
- Classification:Immunoglobulin heavy locus at 14q32.33
- VEGA ID:OTTHUMG00000152325
- Identity:HGNC:31499
- Gene:MIR1-1
- Long gene name:microRNA 1-1
- Synonyms:hsa-mir-1-1
- Discovery year:2004-04-23
- Entrez gene record:406904
- RefSeq identity:NR_029780
- Classification:MicroRNA MIR1/206 family
- Identity:HGNC:50820
- Gene:TRUND-NNN8-1
- Long gene name:tRNA-undetermined (NNN) 8-1
- Synonyms gene name:transfer RNA-undetermined (NNN) 8-1
- Discovery year:2014-06-20
- Entrez gene record:103482528
- Pubmed identification:18984615
- Identity:HGNC:50819
- Gene:TRUND-NNN7-1
- Long gene name:tRNA-undetermined (NNN) 7-1
- Synonyms gene name:transfer RNA-undetermined (NNN) 7-1
- Discovery year:2014-06-20
- Entrez gene record:103482531
- Pubmed identification:18984615
- Identity:HGNC:5760
- Gene:IGKV1OR2-1
- Long gene name:immunoglobulin kappa variable 1/OR2-1 (pseudogene)
- Synonyms gene name:immunoglobulin kappa variable 1/OR-1, immunoglobulin kappa variable 1/OR-1 pseudogene, immunoglobulin kappa variable 1/OR-1 (pseudogene)
- Synonyms:IGKV1/OR-1, IGKV1/OR2-1,
- Discovery year:2000-04-18
- Entrez gene record:3531
- Pubmed identification:2853098
- Classification:Immunoglobulin kappa (IGK) orphons
- VEGA ID:OTTHUMG00000155081
- Gene symbolTRC-GCA21-1, TRSUP-TTA2-1, TRA-AGC21-1, TRN-GTT21-1, TRL-CAG2-1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1
- IsotypeAntibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
- Short nameCytokeratin Antigen 21-1 (CYFRA21-1) CLIA Kit, 96
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- antigen
- Techniqueantigen, antigenes
- Alternative techniqueantigenes, kits