- Catalog numberEKU09449
- Product nameRat Matrix Metalloproteinase 8 (MMP8) CLIA Kit, 96 well plate
- Size5x96T
- PriceAsk For Price
- Verified reactivityRattus norvegicus (Rat)
- Detection limits78.12-5000 pg/mL
- Sensitivity limit2,48 pg/mL
- Samples to be analyzedSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
- Assay duration2 hours, 40min
- Verified applicationsCLIA
- Assay classSandwich
- ELISA detectionChemiluminescent
- Protein numberO88766
- Alternate protein numberPlease refer to SwissProt
- Gene number63849
- Gene nameMatrix Metalloproteinase 8
- Alternate gene nameCLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase
- Estimated production time7-11 business days
- Shipping requirementsBlue ice
- Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Use for8 months
- ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 8 (MMP8). No significant cross-reactivity or interference between Matrix Metalloproteinase 8 (MMP8) and analogues was observed.
- ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
- Assay principleThe microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 8 (MMP8). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 8 (MMP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 8 (MMP8) level in the sample or standard.;
- ProtocolPlease see ELISA's datasheet, otherwise contact us
- Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 8 (MMP8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 8 (MMP8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
- ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
- Research main areaEnzyme & Kinase;Infection immunity;Cardiovascular biology;
- WarningsDo not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- NotesFor research use only. Not for diagnostic procedures.
- Hyperlink
- Alternative to gene target
- [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
- Gene targetMatrix Metalloproteinase 8 MMP8 CLIA Kit 96
- Identity:HGNC:7175
- Gene:MMP8
- Long gene name:matrix metallopeptidase 8
- Synonyms gene name:matrix metalloproteinase 8 (neutrophil collagenase)
- Discovery year:1991-09-11
- Entrez gene record:4317
- RefSeq identity:NM_002424
- Classification:M10 matrix metallopeptidases
- VEGA ID:OTTHUMG00000167587
- Gene symbolMMP8
- HostRat
- Short nameMatrix Metalloproteinase 8 (MMP8) CLIA Kit, 96
- Alternative techniquekits