gentaur logogentaur logo
x

MYCOBACTERIUM TUBERCULOSIS HEAT SHOCK PROTEIN 70 Protein

  • Catalog number
    EKU08776
  • Product name
    Mouse Selectin, Endothelium (SELE) CLIA Kit, 96 well plate
  • Size
    10x96T
  • Verified reactivity
    Mus musculus (Mouse)
  • Detection limits
    27.4-20,000 pg/mL
  • Sensitivity limit
    11,6 pg/mL
  • Samples to be analyzed
    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Assay duration
    2 hours, 40min
  • Verified applications
    CLIA
  • Assay class
    Sandwich
  • ELISA detection
    Chemiluminescent
  • Protein number
    Please refer to Uniprot
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    Please refer to GenBank
  • Gene name
    Selectin, Endothelium
  • Alternate gene name
    CD62E; CD62-E; E-Selectin; ELAM; ELAM1; ESEL; SEL-E; E-LAM; E-LAM1; E-SEL; LECAM2; Endothelial Leukocyte Adhesion Molecule 1; CD62 Antigen-Like Family Member E
  • Estimated production time
    7-11 business days
  • Shipping requirements
    Blue ice
  • Storage recommendation
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    8 months
  • ELISA s specificity
    This assay has high sensitivity and excellent specificity for detection of Selectin, Endothelium (SELE). No significant cross-reactivity or interference between Selectin, Endothelium (SELE) and analogues was observed.
  • ELISA s cross reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    The microplate provided in this kit has been pre-coated with an antibody specific to Selectin, Endothelium (SELE). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Selectin, Endothelium (SELE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Selectin, Endothelium (SELE) level in the sample or standard.;
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Selectin, Endothelium (SELE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selectin, Endothelium (SELE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA s stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
  • Warnings
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Hyperlink
    Hyperlink
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
    • [ "selectin E", "CD62E and ELAM and ELAM1 and ESEL and LECAM2", "SELE and IDBG-104701 and ENSG00000007908 and 6401", "oli this GO saccharide binding", "Extracellular", "Sele and IDBG-202283 and ENSMUSG00000026582 and 20339", "SELE and IDBG-631886 and ENSBTAG00000007307 and 281484" ]
  • Gene target
    Selectin Endothelium SELE CLIA Kit 96
    • Gene info
  • Gene symbol
    SELE
  • Host
    mouse
  • Short name
    Mouse Selectin, Endothelium (SELE) CLIA Kit, 96
  • technique filter
    • Mouse
  • Technique
    Mouse, mouses
  • Alternative technique
    murine, kits