High throuput assay system for real-time gene expression monitoring of living cells.

WSL-1565 Kronos HT is the high throughput support model of the assay system that measure the gene expression of living cells in real-time with the bioluminescence of luciferase. Kronos Dio, which is a precedent model compatible with 35 mm dish, has been used as a real-time luminescence measurement system with cell culturing function, but this time a new lineup of model compatible with multiwell plate measurement is added. With this new product Kronos HT, we succeeded the know-how cultivated in the precedent model in order to measure the luminescence while culturing the cells, and further strengthened functions and performance for high throughput measurement. Please consider adopting Kronos HT for high-throughput measurement in biological clock analysis, change in drug response with time, cytotoxicity test etc.

Biological clock, organ clock and cell clock... 

• Cell culture function with temperature contol, humidifying and controlling CO2 concentration
• 24 and 96 well plate format
• Scan 2 plates with 2 detectors (PMTs)
• It does not shake or vibrate cells by scanning with detector moving and cuture plates not moving.

Reporter assay in real time with culturing cells

Reporter assay is used to measure the transcriptional activity of gene transcription regulatory sequences such as promoters, for researches on drug response and gene expression control, evaluation of gene transfer efficiency, and so on. Among them, the method of measuring the amount of bioluminescence using bioluminescent enzyme luciferase using a device called a luminometer is superior in terms of high sensitivity and low background and is widely used. Usually, transcription activity at a certain point (end point) is measured, but the assay is also becoming used for tracking transcription activity over time such as stimulation response, time course of transgene expression level, periodic variation of biological clock gene. Kronos HT is a system to optimize for such assays with the ability to monitor the bioluminescence over time as cells are cultured. 

Chronomedicine, research progresses in the world ...

High sensitive and low noise detection by cooling of detectors (PMTs)

Compatible with multicolor luciferase assay

Easy setting with software on Windows PC, and displaying and saving measurement data in real time.

Peristaltic pump with high flow precision 3-channel type with long tube life

A Fusion of Over 60 years of Technological Accumulation and Innovation Complete tube set with one-touch operation Combining our 60 years of development experience since our founding with the latest technology, we have evolved into an innovative, compact, highperformance digital pump that has been refined and combines ease of use and high performance. Adopting a new roller system, it achieves highly reproducible flow accuracy and stability while reducing the effects of pulsation. Furthermore, in pursuit of intuitive and simple operability, the convenience of attaching and detaching tubes with one hand and with a single touch, and the touch panel that anyone can easily use. PeristaQuantumPump is an innovative fluid control solution that combines ease of use and performance for users who demand reliability and convenience.

▶ Automatic operation enables timer liquid feeding and fixed volume liquid feeding.

▶ Compatible with various solutions by using olefin tube with high chemical resistance and durability

▶ Adoption of stepping motor improves durability and extends lifespan

▶ One-touch tube attachment/detachment allows quick replacement

▶ Achieves fixed-quantity liquid transfer with high flow rate accuracy and flow stability

▶ Easy calibration with semi-automatic operation

The “WSP-3310 peristaQuantumPump” operates within a wide range of 0.01 rpm to 50 rpm (a maximum adjustment range of 5,000 times), where the flow rate is proportional to the pump’s rotation speed, exhibiting consistent linearity (R-squared coefficient = 1). In long-term liquid delivery experiments conducted at set flow rates from 1 mL/min to 40 mL/min, excluding the initial 1 hour until the tube stabilized, it was confirmed that very stable flow control is possible with a CV value of 1.07%. Additionally, upon evaluating the effects of liquid temperature, it was demonstrated that stable liquid delivery can be achieved under consistent temperatures. These results indicate that the peristaQuantumPump can achieve high precision and stable liquid delivery across a broad range of flow rates.

Innovative technology

Fluid control with PeristaQuantumPump

PeristaltQuantumPump, likes other tube-based pumps, utilize the elasticity of the tube to transport fluids.

The core mechanism of this pump involves the continuous compression of the tube by rollers moving along it. This action, combined with the pressure exerted and the tube’s restorative force, propels the fluid. PeristaltQuantumPump employs self-propelled rollers. When the main shaft (A) rotates, the surrounding rollers (B) move in the direction of the shaft’s rotation. Upon contacting the tube, rollers (B) rotate in the opposite direction of the main shaft (A) while compressing the tube. As the tube passes through the compression section, a negative pressure is created inside, drawing in liquid, and the rotational motion expels the liquid on the outlet side. Since the liquid only travels inside the tube and does not come into contact with other parts of the pump, the risk of contamination is extremely low. Additionally, simply replacing the tube allows for easy cleaning and maintenance. The elasticity of the tube also enables the transport of viscous liquids or liquids containing particles. PeristaltQuantumPump offers precise flow control and high reproducibility, making them suitable for a wide range of applications in the medical, research, and industrial fields.

High performance digital pump with high rigidity mechanism

This is the original peristaQuantumPump, a condensation of Ato’s technological essence. With 12 rollers minimizing the effects of pulsation, it achieves reproducible liquid delivery with high flow rate precision. Proper compression control extends the tube’s lifespan to over two weeks, even with continuous operation at maximum flow rate, while maintaining flow rate precision and stability. Utilizing three flow paths, it allows for the delivery of multiple targets under identical conditions with even flow rates or the delivery of multiple solutions at the same flow rate. Moreover, with the PQP support program, external control can be easily programmed. As a fluid control solution ideal for applications requiring precise flow control, it is a product that professionals prioritizing reliability can depend on.

▶ Achieved low pulsation flow with 12 rotating roller system

▶ Achieving high flow rate accuracy through quantitative flow rate stability and high flow rate reproducibility

▶ Longer tube life with appropriate discharge pressure control

▶ Easy touch panel operation and settings

▶ BioSafe sterile, contamination-free, and hygienic liquid delivery

▶ Improve productivity with external control, foot switches, and remote control

WSE-5300 Printgraph CMOSI is High resolution 6M pixels monochrome CMOS camera applied with touch panel control system. Touch panel interface is reflecting ATTO original knowhow which is closing user-friendly for 30 years sales. Detecting by UV light source which DNA(EtBr) and detection CBB pattern with white flat LED light source. ATTO original「CyanRed Epi」is switchable LED light which are Cyan LED and Red LED. Cyan LED is available to detect a fluorescence in both blue and green wavelenth. Detetable EtBr which is difficult to detect in Blue LED. However, ATTO made its possible for UV free Gel Documentation System with CyanRed Epi. Save an image to USB memory directly or print out immediately with UP-X898MD thermal printer.

PAGEL (precast gels) are ready-to-use polyacrylamide gels designed for efficient electrophoresis applications. They offer consistent quality and reliable performance, saving time in the lab while ensuring accurate separation of proteins and nucleic acids for analysis.

Applications & Use

• Protein analysis using ( E- type gels)
• Second-dimension separation in 2D electrophoresis with (E-D type gels)
• SDS-PAGE and Native-PAGE for protein separation
• DNA analysis, including PCR product detection
• High-throughput screening
• Educational use in training students and researchers.

Product Highlights

• Compact footprint: 90 mm × 83 mm – optimized for bench space
• Reliable performance: Delivers stable and reproducible separation
• High-quality glass plates: Excellent thermal stability and gel uniformity
• Convenient handling: Easy setup and gel removal
• Versatile gel options: Wide range of acrylamide concentrations, well formats, and gels for 2D applications
• System compatibility:
• Designed for use with ATTO mini gel systems:
• WSE-1150P PageRunAce
• WSE-1165 Mini-Slab

Note: Uses 5 mm thick glass plates – compatible PAGEL plate holders are required.

The WSE-1165 Mini-Slab by ATTO Corporation is a compact vertical electrophoresis system designed for high-speed, high-resolution protein and nucleic acid separation. It's particularly suited for laboratories with limited bench space, educational settings, and researchers seeking efficient and reliable electrophoretic analysis.

🔬 Key Features

• Compact Design: The unit's small footprint (165 mm W × 99 mm D × 134 mm H) makes it ideal for laboratories with space constraints.
• High-Speed Electrophoresis: Capable of completing runs in as little as 20–25 minutes when used with WSE-7065 EzRunMOPS buffer, facilitating rapid analysis.
• Built-In Power Supply: The integrated power supply simplifies setup and operation, eliminating the need for external equipment.
• Automatic Polarity Switching: Ensures consistent results by automatically designating the lower electrode as the anode, regardless of power supply connection orientation.
• Leak-Proof Operation: Features a specialized sealing mechanism with flexible, wave-shaped materials to prevent buffer leakage and maintain gel integrity.
• Temperature Uniformity: Utilizes a lower buffer solution to achieve consistent temperature across both sides of the gel, reducing the "smiling" effect and enhancing band resolution.
• User-Friendly Gel Handling: The system's design allows for easy installation and removal of gel plates, streamlining the electrophoresis process.

🧪 Applications

• Protein Analysis: Suitable for SDS-PAGE and Native-PAGE, enabling the separation and analysis of proteins under denaturing and non-denaturing conditions.
• Nucleic Acid Separation: Effective for DNA electrophoresis, including the analysis of PCR products.
• Purity Assessment: Facilitates the determination of protein purity and molecular weight estimation
• Educational Use: Ideal for training students and beginners in electrophoresis techniques due to its straightforward operation and reliable performance.

📐 Specifications

• Gel Size: 90 mm (W) × 80 mm (L) × 1 mm (T)
• Buffer Volume: Approximately 400–650 mL
• Material: Constructed from durable acrylic and polycarbonate materials
• Power Supply: 100–240V, 50/60Hz, 50VA
• Electrophoresis Modes:
• High Mode: ~35 minutes (or ~20 minutes with EzRunMOPS buffer)
• Standard Mode: ~70 minutes
• Low Mode: ~140 minutes for proteins; ~70 minutes for DNA

🔗 Compatibility & Accessories

• Gel Casting: Compatible with AE-6401 1 mm Dual Mini Gel Cast and WSE-1190 Multi Mini-Slab Gel Cast for hand-cast gels.
• Precast Gels: Supports ATTO's e-PAGEL and e-PAGEL HR precast gels, offering convenience and consistency.
• Power Supply: While the unit includes a built-in power supply, it is also compatible with the PageRunAce Power Supply for enhanced control.

The WSE-1165 Mini-Slab system combines efficiency, reliability, and user-friendly features, making it a valuable tool for various electrophoretic applications in research and educational laboratories.

Western blotting is a method used to detect specific proteins in a sample.It involves separating proteins by size, transferring them to a membrane, and using antibodies to identify the target protein.This technique is fundamental in molecular biology and biochemistry for analyzing protein expression and modifications.

1. Transfer Buffers

• EzBlot (AE-1460): A three-component transfer buffer optimized for semi-dry blotting, facilitating efficient protein transfer.
• EzFastBlot (AE-1465): A methanol, acid, and detergent-free buffer designed for rapid semi-dry transfers, ensuring safety and speed.
• EzFastBlot HMW (WSE-7210): Tailored for high molecular weight proteins (>200 kDa), providing efficient transfer without harsh chemicals.
• EzRun TG (WSE-7055): A buffer formulated for tank blotting systems, ensuring consistent protein transfer.

2. Blocking Reagents

• EzBlock Chemi (AE-1475): A non-protein blocking agent that minimizes background noise, suitable for Western blotting, ELISA, and immunostaining.
• EzBlock BSA (AE-1476): A bovine serum albumin-based blocker commonly used in immunochemical analyses to prevent nonspecific binding.

3. Wash Buffers & Additives

• EzTBS (WSE-7230): A Tris-buffered saline solution used as a wash buffer in Western blotting protocols.
• EzTween (WSE-7235): An additive for TBS or PBS buffers, enhancing washing efficiency and reducing background.

4. Membrane Staining

• EzStain AQuaMenm (WSE-7160): A total protein stain kit for transfer membranes, allowing visualization of protein bands post-transfer.

5. Stripping Reagents

• EzReprobe (WSE-7240): A high-performance stripping reagent that effectively removes primary and secondary antibodies from membranes, enabling multiple reprobing cycles.

⚙️ Blotting Systems & Accessories

ATTO provides a range of blotting systems and accessories to complement their reagents:

• HorizeBLOT Series (e.g., WSE-4025, WSE-4045): Semi-dry blotting systems designed for efficient protein transfer.
• PowerdBlot Series (e.g., WSE-4125, WSE-4115): Blotting units with integrated power supplies, streamlining the transfer process.
• P plus Membranes (e.g., WSE-4050 to WSE-4054): PVDF membranes available in various sizes, compatible with ATTO's blotting systems.
• Filter Papers (e.g., CB-06A to CB20A): High-quality blot absorbent papers tailored for different gel sizes.

ATTO's Western blotting solutions are designed to provide researchers with reliable and efficient tools for protein analysis.

The WSE-1710 Submerge-Mini is a compact and versatile agarose gel electrophoresis system designed for efficient DNA and nucleic acid analysis. It's particularly suitable for applications such as PCR fragment analysis, genotyping, and DNA purity assessment.

🔬 Key Features

• High-Speed Electrophoresis: Supports multiple constant voltage settings—18V, 25V, 35V, 50V, 70V, 100V, and up to 135V—allowing rapid separation of DNA fragments.
• Extended Timer Function: Programmable up to 99 hours and 99 minutes, with an alarm to signal completion, ensuring precise control over electrophoresis duration.
• Safety Features: Equipped with a lid interlock system that halts current flow when the lid is opened, preventing accidental exposure to electrical hazards.
• Versatile Gel Tray Options: Includes three gel tray sizes—60×60mm, 120×60mm, and 120×120mm—to accommodate various sample volumes and analysis needs.
• Heat-Resistant Components: Gel trays and combs are constructed from materials that withstand temperatures ranging from -40°C to 135°C for trays and -20°C to 115°C for combs, ensuring durability and compatibility with various protocols.
• User-Friendly Design: The system features an intuitive interface and clearly labeled components, making it accessible for both novice and experienced users.

📐 Specifications

• Voltage Output: 18V / 25V / 35V / 50V / 70V / 100V / 135V
• Timer: Up to 99 hours and 99 minutes
• Gel Tray Sizes: 60×60mm, 120×60mm, 120×120mm
• Buffer Volume: Approximately 200–230 mL
• Power Input: AC100–120V, 50/60Hz
• Dimensions: 190 (W) × 130 (D) × 60 (H) mm
• Weight: Approximately 0.45 kg

🧪 Applications

• DNA Fragment Analysis: Ideal for separating PCR-amplified DNA fragments and restriction enzyme digests.
• Genotyping: Useful in identifying genetic variations and mutations.
• DNA Purity Assessment: Allows evaluation of DNA sample integrity and purity.
• Educational Use: Suitable for teaching laboratories due to its straightforward operation.

🔧 Purpose:

The PQP Control Software is an external PC-based control application for operating up to four PeristaQuantumPumps (WSP-3300 / WSP-3310). It enables users to manage complex liquid dispensing operations with precision and flexibility.

🧪 Key Features of the Software:

• Three flow modes:

1. Manual Flow (Continuous, Time, Volume modes)
2. Calibration
3. Programmed Flow (up to 20 steps with repeat functions)

• Control Options:
• Flow rate (mL/min or mL/h)
• Flow direction (CW/CCW)
• Start/Stop times
• Gradient delivery
• Simultaneous control of multiple pumps
• File saving and loading of programmed steps
• User Interface:
• Intuitive design with drag-and-drop programming
• Profile display of flow sequences
• Copy, paste, delete steps across pumps

💻 System Requirements:

• Windows 10/11 (64-bit)
• 8 GB RAM
• Display: 1920 × 1080
• Language options: Japanese / English

💡 Pump Models & Specs:

• WSP-3300: Triple-channel, low pulsation, suitable for stable high-precision flow
• WSP-3310 / 3310-2: Single or dual-channel, compact and durable with touch panel

🧵 Tubing Information:

• Olefin tubes with Luer connectors (2/4 mm or 4/6 mm)
• Chemical resistance data provided for acids, solvents, and alkalis
• Designed for high flow accuracy and durability

📦 Included Products / Codes:

• Pumps, software, tubing, additional pump heads, and foot switches
• Product codes (e.g., 1221413 for the software)

⚠️ Note:

These devices are research instruments only and not for medical use.

🔍 Why Normalize Western Blots?

Western blotting involves many variables. Even if the same amount of sample is applied to each lane, differences in sample type, pipetting accuracy, antibody reaction, or transfer method can affect the results. Normalization corrects these inconsistencies, allowing reliable comparison between samples or blots.

⚠️ Limitations of Housekeeping Proteins

Traditionally, HKPs like GAPDH, actin, or tubulin are used as internal controls. However, their expression levels can vary between tissue types, developmental stages, or under different experimental conditions. Moreover, their high abundance can mask the true expression level of the target protein, leading to incorrect conclusions.

✅ Advantages of Total Protein Normalization (TPN)

Using total protein as a reference offers a more stable and universal approach. It reflects the actual amount of protein transferred per lane, reducing the risk of error caused by variation in individual protein expression or antibody reactivity. TPN is compatible with modern labeling techniques, such as fluorescent dyes and stain-free gels.

🔧 How to Perform TPN

Start by labeling protein samples using fluorescent reagents like EzLabel FluoroNeo, or use stain-free gels. Avoid adding reducing agents before labeling, as they interfere with the reaction.

Run the samples on SDS-PAGE and transfer the proteins onto a PVDF membrane. Fluorescence-compatible, low-background membranes are preferred. After transfer, image the membrane to detect total protein, and then proceed with blocking and antibody-based detection of the target protein.

Use software such as CS Analyzer 4 to analyze the images. Define each lane and band, subtract the background, and then calculate the normalized intensity of the target protein based on the corresponding total protein signal in the same lane.

💻 Normalization Method

To calculate the normalized value of a target protein, divide the band intensity of the target protein by the total protein reference value for that lane. This value is then compared against a reference lane set as “1” to determine relative expression levels.

📈 Interpreting Results

Normalization using total protein usually shows consistent and expected trends, such as clear dilution patterns or accurate changes in expression. In contrast, HKP-based normalization can sometimes give misleading results, especially if HKPs are overexpressed or affected by experimental conditions.

🔬 Tools & Reagents Used

The method commonly uses reagents like EzLabel FluoroNeo for protein labeling and EzApply for sample preparation. Transfer buffers like EzFastBlot ensure fast and efficient protein transfer. PVDF membranes with low fluorescence background are ideal for imaging. Stain-free gels or dyes like Ponceau and CBB can also be used for total protein detection.

🔄 Alternative Detection Approaches

Other than fluorescent labeling, stain-free gels can also visualize total protein through UV activation. Membranes stained with CBB or Ponceau provide visible detection, although they may have lower sensitivity compared to fluorescent methods.