-
Available ordering format
Lyophilized in PBS pH 7.4
-
Immunogen
BSA-conjugated synthetic peptide derived from the Lhca4 protein of Arabidopsis thaliana (At1g45474). This sequence is highly conserved in Lhca5 proteins of angiosperms (monocots and dicots) and gymnosperms.
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Total IgG
-
How to reconstitute
For reconstitution add 200 µl of sterile water
-
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
western blot (WB)
-
Connected products
AS01 005 Lhca1 | PSI type I chlorophyll a/b-binding proteinAS01 006 Lhca2 | PSI type II chloropyll a/b-binding proteinAS01 007 Lhca3 | PSI type III chlorophyll a/b-binding proteinAS01 008 Lhca4 | PSI type IV chlorophyll a/b-binding proteinLHC available antibodies against pigment-binding proteinsPlant protein extraction buffer Secondary antibodies
-
Recommended dilutions for use
1: 1000- 1: 3000 with standard ECL (WB)
-
Molecular weight expected аpparent
27.8 | 23 for Arabidopsis thaliana
-
Verified reactivity
Arabidopsis thaliana, Bryopsis corticulans, Hordeum vulgare, Spinacia oleracea
-
Possible reactivity
monocots and dicots
-
No reactivity
Chlamydomonas reinhardtii
-
Supplementary information
Protein is processed into mature form (Jansson 1999).
-
References
Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.
-
Scientific context
Lhca5 is a low abundant chlorophyll a/b-binding protein that has been found to associate with Photoystem I in sub-stoechiometric amounts. Unlike the four main Lhca-types (Lhca1-4) Lhca5 seems to be able to associate with PSI or the LHCI at varying positions. The regulation pattern for the expressino of the Lhca5 gene differs from the standard Lhc pattern as expression seems to be positively regulated under light and cold stress conditions.
-
Notes
More information on the Lhca5 localization within the LHCI antenna can be found in Lucinski et al. (2006) Lhca5 interaction with plant photosystem I. FEBS Letters 580:6485-6488
-
Protein number
Q9C639
-
TAIR number
AT1G45474
-
