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Available ordering format
Lyophilized
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Immunogen
Purified Rabbit IgG, whole molecule.
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified goat IgG.
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How to reconstitute
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
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Storage condition
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
ELISA (ELISA) , Western Blot (WB), Immunohistochemistry (IHC)
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Connected products
AS09 607 | Goat anti-rabbit IgG (H&L) ALP conjugatedAS09 608 | Goat anti-rabbit IgG (H&L) biotin conjugatedAS10 668 | Goat anti-Rabbit IgG (H&L) HRP conjugated, min.cross-reactivity to bovine/Human/mouse IgG/serum
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Recommended dilutions for use
1: 50 000 -1: 90 000 (ELISA), 1 : 75 000 with enhanced ECL and 1: 25 000 with standard ECL (WB), 1: 500 -1: 5000 (IHC)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Based on IEP, this antibody reacts with: rabbit IgG heavy chainslight chains on all rabbit immunoglobulins
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Possible reactivity
to be determined
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No reactivity
No confirmed exceptions from predicted reactivity known in the moment.
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Supplementary information
No reactivity is observed to non-immunoglobulin rabbit serum.
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References
Datta et al. (2016). Glutathione Regulates 1-Aminocyclopropane-1-Carboxylate Synthase Transcription via WRKY33 and 1-Aminocyclopropane-1-Carboxylate Oxidase by Modulating Messenger RNA Stability to Induce Ethylene Synthesis during Stress. Plant Physiol. 2015 Dec;169(4):2963-81. doi: 10.1104/pp.15.01543. Epub 2015 Oct 13.Barahimipour et al. (2016). Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker. Plant Mol Biol. 2016 Mar;90(4-5):403-18. doi: 10.1007/s11103-015-0425-8. Epub 2016 Jan 8.MacLellan et al. (2015). Chaperone roles for TMAO and HSP70 during hyposmotic stress in the spiny dogfish shark (Squalus acanthias). J Comp Physiol B. 2015 Jun 7.Giuntoli et al. (2014). A trihelix DNA binding protein counterbalances hypoxia-responsive transcriptional activation in Arabidopsis. PLoS Biol. 2014 Sep 16;12(9):e1001950. doi: 10.1371/journal.pbio.1001950. eCollection 2014.Zubo et al. (2014). Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria. Mitochondrion. 2014 Mar 31. pii: S1567-7249(14)00037-3. doi: 10.1016/j.mito.2014.03.011Ivanov et al. (2014). SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1. Plant Cell. 2014 Mar 4.
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Scientific context
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
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Notes
Antibody is provided in: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% BSA (w/v), Protease IgG free, 0.1 % (v/v) Kathon CG.Affinity purified antibody is > 95 % pure, according to SDS-PAGE.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
