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Goat, IgM

  • Catalog number
    AS10 804
  • Product name
    Goat, IgM
  • Size
    1 mg
  • Price
    Ask For Price
  • Contact us
    Goat, IgM
  • Available ordering format
    Lyophilized
  • Immunogen
    Purified human IgG
  • Raised in
    Goat
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified goat IgG
  • How to reconstitute
    For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
  • Storage condition
    Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Verified applications
    ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
  • Connected products
    antibodies to human immunoglobulins
  • Recommended dilutions for use
    optimal working dilution should be determined by the investigator
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    human IgG Fc (two heavy chains with constant domains)
  • Possible reactivity
    human IgG Fc (two heavy chains with constant domains)
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on human immunoglobulins or human IgA and IgM based on immunoelectrophoresis.
  • References
    to be added when available
  • Scientific context
    Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
  • Notes
    HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Hyperlink
    Hyperlink
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    IgM, IgM
  • isotype filter
    • IgM
  • Short name
    Goat, IgM
  • technique filter
    • Goat
    • IgM
  • Technique
    Goat, IgM, goats, IgMs
  • Alternative technique
    caprine, immunoglobulin-m