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Goat, F(ab)'2 fragment, FITC conjugated, min. cross-reactivity to bovine,mouse,rabbit serum

  • Catalog number
    AS10 1015
  • Product name
    Goat, F(ab)'2 fragment, FITC conjugated, min. cross-reactivity to bovine,mouse,rabbit serum
  • Size
    0.5 mg
  • Available ordering format
    Liquid
  • Immunogen
    to be determined
  • Raised in
    Goat
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified IgG, F(ab)'2 fragment
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    Store at 2-8°C. Shelf life of this product is one year from date of receipt.
  • Verified applications
    Contact Gentaur support center
  • Connected products
    other fluorescent dye conjugated antibodies to human IgG
  • Recommended dilutions for use
    1:20-1: 2000 for immunohistochemistry (IHC),1:50-1: 5000 for immunocytochemistry (ICC). (ICC).
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    to be determined
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    Refer to PubMed
  • Scientific context
    Goat anti-human IgG (H&L) is a FITC (fluorescein-5-isothiocyanate) conjugated secondary antibody F(ab)'2 fragment, which binds to all human IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed to bovine,mouse,rabbit serum and its purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
  • Notes
    This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Hyperlink
    Hyperlink
  • Gene target
    F ab '2 conjugated min cross
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
  • Isotype
    Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
  • Short name
    Goat, F(ab)'2 , FITC conjugated, min. cross-reactivity ,mouse rabbit serum
  • Label
    FITC
  • label filter
    • FITC
  • technique filter
    • Rabbit
    • Mouse
    • serum
    • FITC
    • Goat
  • Technique
    Rabbit, Mouse, serum, FITC, Goat, antibody Conjugates, Fluorescein, goats, mouses
  • Alternative technique
    rabbit-anti, sera, murine, caprine, fluorescine
  • Tissue
    serum
  • tissue filter
    • serum