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Goat, F(ab)'2 fragment, FITC conjugated

  • Catalog number
    AS10 1011
  • Product name
    Goat, F(ab)'2 fragment, FITC conjugated
  • Size
    0.5 mg
  • Available ordering format
    Liquid
  • Immunogen
    to be determined
  • Raised in
    Goat
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified IgG, F(ab)'2 fragment
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    Store at 2-8°C. Shelf life of this product is one year from date of receipt.
  • Verified applications
    Contact Gentaur support center
  • Connected products
    other fluorescent dye conjugated secondary antibodies to rabbit IgG
  • Recommended dilutions for use
    1:20-1: 2000 for immunohistochemistry (IHC),1:50-1: 5000 for immunocytochemistry (ICC) and flow cytometry.
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    to be determined
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    Refer to PubMed
  • Scientific context
    Goat anti-rabbit IgG (H&L), F(ab)'2 fragment of FITC (fluorescein-5-isothiocyanate) conjugated secondary antibody which binds to rabbit IgG in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is >90% based on SDS-PAGE and is affinity purified on solid phase rabbit IgG. Antibody solution may contain small amounts of intact IgG.
  • Notes
    This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Hyperlink
    Hyperlink
  • Gene target
    F ab '2 conjugated
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
  • Short name
    Goat, F(ab)'2 , FITC conjugated
  • Label
    FITC
  • label filter
    • FITC
  • technique filter
    • FITC
    • Goat
  • Technique
    FITC, Goat, antibody Conjugates, Fluorescein, goats
  • Alternative technique
    caprine, fluorescine