gentaur logogentaur logo
x

Donkey, IgG

  • Catalog number
    AS10 1008
  • Product name
    Donkey, IgG
  • Size
    1 mg
  • Price
    Ask For Price
  • Contact us
    Donkey, IgG
  • Available ordering format
    Lyophilized
  • Immunogen
    to be determined
  • Raised in
    Donkey
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified IgG
  • How to reconstitute
    For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
  • Storage condition
    Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Verified applications
    Contact Gentaur support center
  • Connected products
    other HRP conjugated antibodies to rabbit IgG
  • Recommended dilutions for use
    Needs to be determined by end user for all immunoassay applications.
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    to be determined
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    Petersen and Andersen (2014). Simultaneous isolation of mRNA and native protein from minute samples of cells. Biotechniques. 2014 May 1;56(5):229-37. doi: 10.2144/000114165. eCollection 2014.
  • Scientific context
    Donkey anti-rabbit IgG (H&L) is a secondary antibody, HRP conjugated, which binds to all rabbit IgGs in immunological assays. Antibody is specially adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG. Antibodies are affinity purified using solid phase rabbit IgG.
  • Notes
    This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Hyperlink
    Hyperlink
  • Host
    Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
  • Isotype
    IgG, IgG
  • isotype filter
    • IgG
  • Short name
    , IgG
  • technique filter
    • IgG
  • Technique
    IgG, IgGs
  • Alternative technique
    immunoglobulins