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Available ordering format
Lyophilized
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Immunogen
overexpressed cytosolic fructose 1,6 bisphosphatase (cFBPase) derived from the sequence from Arabidopsis thaliana cFBPase Q9MA79
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 100 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
collection of other antibodies which can be used as markers of cytoplasmPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 5 000 with standard ECL (WB)
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Molecular weight expected аpparent
45 | 37 kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana, Brassica napus, Macroptilium atropurpureum, Pinus silvestris, Oryza sativa, Petunia hybrida, Solanum tuberosum, Zea mays cellular compartment marker of cytoplasm in photosynthetic tissues
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Possible reactivity
dicots including Pisum sativum, Capsella rubella, Ricinus communis, Glycine max, Phaseolus vulgaris, Vitis vinifera, Spinacia oleracea and monocots and tress: Populus trichocarpa
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No reactivity
Chlamydomonas reinhardtii
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Supplementary information
for image of detection please refer to Strand et al. 2000. This antibody does not react with chloroplastic form of FBPase.Will this antibody be good as a cytosolic (non-microsomal control) in Arabidopsis thaliana roots? Although it has never been tested there is every likelihood that cFBPase will be expressed at reasonable levels even in roots. Even though the biosynthetic flux through to Sucrose may not be high as in mesophyll cells, central metabolism will still be active in young roots and the Sucrose etc being supplied externally still needs to be utilised.
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References
de Michele et al. (2016). Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings. J Proteome Res. 2016 Mar 4;15(3):900-13. doi: 10.1021/acs.jproteome.5b00876. Epub 2016 Feb 4. Li et al. (2015). Three SAUR proteins SAUR76, SAUR77 and SAUR78 promote plant growth in Arabidopsis. Sci Rep. 2015 Jul 24;5:12477. doi: 10.1038/srep12477. Hu et al. (2015). Site-specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis. Plant Physiol. 2015 Feb 19. pii: pp.00026.2015. Zhang et al. (2014). Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014 Aug 27. pii: S0014-5793(14)00621-8. doi: 0.1016/j.febslet.2014.08.019. Liu et al. (2014). SCFSLF-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida. Front. Genet., 22 July 2014 | doi: 10.3389/fgene.2014.00228 Chen et al. (2014). A two-locus interaction causes interspecific hybrid weakness in rice. Nat Commun. 2014 Feb 21;5:3357. doi: 10.1038/ncomms4357.
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Scientific context
Fructose-1,6 bisphosphatase (FBPase) (EC=3.1.311) is one of the regulatory enzymes in the sucrose biosynthetic pathway. In non-photosynthetic tissues, it regulates the rate of gluconeogenesis. In photosynthetic tissues, two FBPase isozymes (chloroplastic and cytosolic) play key roles in carbon assimilation and metabolism. In photosynthetic tissues cFBPase (cytosolic fructose 1,6 bisphosphatase) converts triose phosphates from the chloroplast to sucrose during light hours. Alternative name: D-fructose-1,6-bisphosphate 1-phosphohydrolase
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Notes
Kinetic and allosteric properties of the plant cytosolic FBPase are remarkably similar to the mammalian and yeast FBPase, but differ greatly from those of the chloroplastic FBPase. The antibody could detect FBPase from the human COS-7 cell line transfected with FBP1 expressing vector.
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Protein number
Q9MA79
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TAIR number
AT1G43670
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