gentaur logogentaur logo
x

Mouse Monoclonal Anti-B. pertussis Toxin (PTX) IgG negative control for ELISA Kit, IF, Western

  • Catalog number
    6335-100
  • Product name
    Human Recombinant AKR7A2
  • Size
    100 ug
  • Synonyms
    Human Recombinant AKR7A2
  • Alternative names
    Aldo-keto reductase family 7, member A 2, Aflatoxin B1 aldehyde reductase member 2, AFAR, AFAR1, AFB1-AR1, AKR7.
  • Description
    Involved in in the detoxification of aldehydes and ketones
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Biological activity
    Specific activity is approximately 0.25 – 0.3 units/mg.
  • Unit Definition
    Specific activity is approximately 0.25 – 0.3 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.
  • Molecular Weight
    44 kDa
  • Storage Temp
    -20°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Concentration
    0.5 mg/ml
  • Appearance
    Liquid
  • Physical form description
    0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 20% glycerol.
  • Background Information
    Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Also, it has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to Gamma-Hydroxybutyrate in human brain.
  • Amino acid sequence
    MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELE LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Datasheet
    Datasheet
  • Gene target
    AKR7A2
    • Gene info
  • Gene symbol
    AKR7A2
  • Short name
    Recombinant AKR7A2
  • technique filter
    • Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Alternative technique
    rec