Mouse Anti-Mouse CD72.1-FITC

• Catalog number
  MBS673440
• Price
  Please ask
• Size
  5x0.5 mg

• Products_type
  Antibody
• Products_short_name
  [CD72.1 (Lyb-2.1)]
• Products_name_syn
  [Mouse Anti-Mouse CD72.1 - FITC; Mouse Anti-Mouse CD72.1 (Lyb-2.1)]
• Clonality
  Monoclonal
• Clone
  [10-1.D.2]
• Reactivity
  Mouse
• Specificity
  Mouse CD72.1/Lyb-2.1, 45 kDa Murine CD72.1/Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells.^1,2 The 10-1.D.2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing.^1 However, 10-1.D.2 can itself trigger a positive signal by binding CD72.^1-5
• Form
  Fluorescein (FITC) Conjugate
• Storage_stability
  The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! All products should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.
• Tested_application
  Flow Cytometry
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD72  
• Gene symbol
  CD72
• Short name
  Mouse Anti-Mouse CD72 1-FITC
• Technique
  anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
• Host
  Mouse
• Isotype
  Mouse (C57/BL6) IgG2b kappa
• Label
  FITC
• Species
  Mouse, Mouses
• Alternative name
  Mouse Antibody toMouse CD72 molecule.1-fluorecein
• Alternative technique
  murine, antibodies, fluorescine
• Alternative to gene target
  CD72 molecule, CD72b and LYB2, CD72 and IDBG-62668 and ENSG00000137101 and 971, carbohydrate binding, Plasma membranes, Cd72 and IDBG-141026 and ENSMUSG00000028459 and 12517, CD72 and IDBG-633031 and ENSBTAG00000011409 and 783725

Gene info

• Identity
  HGNC:1696
• Gene
  CD72
• Long gene name
  CD72 molecule
• Synonyms gene name
  • CD72 antigen
• Synonyms
  • LYB2
  • CD72b
• Locus
  9p13.3
• Discovery year
  1991-10-04
• Entrez gene record
  971
• Pubmed identfication
  • 2044654
  • 1711157
• RefSeq identity
  • NM_001782
• Classification
  • C-type lectin domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000019870

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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