Mouse anti Rabbit IgG H + L Cy3
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Catalog number
43R-IM001C3
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Price
Please ask
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Size
1 mg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Mouse anti-rabbit IgG (H + L) (Cy3) was raised in mouse using rabbit igG (H & L) ..
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Raised in
Mouse
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Specificity
Rabbit
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
CY3
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Method of Purification
Mouse anti Rabbit IgG (H + L) (Cy3) was purified by affinity chromatography.
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Form Buffer
Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.
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Storage
Store at 4 deg C.
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Shipping conditions
Blue Ice
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Tested for
Please refer to the datasheet
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Mouse anti Rabbit IgG H + L can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This fitzgerald antibody is well suited for fluorescent microscopy.
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Conjugation
cy3 conjugation kit
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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Gene target
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Gene symbol
CALD1, FUT1, L3MBTL2
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Short name
Mouse anti Rabbit IgG H + L
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Technique
Rabbit, Mouse, anti, IgG, antibody to, IgGs, mouses
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Host
mouse, Rabbits
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Isotype
IgG, IgG
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Label
Cy3
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Species
Mouse, Mouses
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Alternative name
Mouse anti Rabbit IgG (H + L) secondary antibody (Cy3)
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Alternative technique
rabbit-anti, murine, antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
caldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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