CD2 FITC, Single-Color Reagent

• Catalog number
  0022
• Price
  Please ask
• Size
  100Tests

• Host Source
  Mouse
• Clone Name
  T6.3
• Product Category
  Flow Cytometry
• Species Reactivity
  Human
• Application
  Flow Cytometry
• Immunogen
  Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by mixed lymphocyte culture.
• Purification Method
  Protein A/G Chromatography
• Shipping Conditions
  Ship at ambient temperature, do not freeze, refrigerate upon arrival
• Storage conditions
  CD2 FITC, Single-Color Reagent should be stored at 4-8ºC. DO NOT FREEZE
• Datasheet link
• Properties
  This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  CD2   Single-Color   Reagent  
• Gene symbol
  CD2
• Short name
  CD2 FITC, Single-Color Reagent
• Technique
  FITC, Fluorescein
• Isotype
  IgG2a
• Label
  FITC
• Alternative name
  CD2 molecule fluorecein, Single-Color Reagent
• Alternative technique
  fluorescine
• Alternative to gene target
  CD2 molecule, LFA-2 and SRBC and T11, CD2 and IDBG-101417 and ENSG00000116824 and 914, protein homodimerization activity, Extracellular, Cd2 and IDBG-179684 and ENSMUSG00000027863 and 12481, CD2 and IDBG-634458 and ENSBTAG00000017256 and 497022

Gene info

• Identity
  HGNC:1639
• Gene
  CD2
• Long gene name
  CD2 molecule
• Synonyms gene
  • SRBC
• Synonyms gene name
  • CD2 antigen (p50), sheep red blood cell receptor
• GenBank acession
  • BC033583
• Locus
  1p13.1
• Discovery year
  1986-01-01
• Entrez gene record
  914
• Pubmed identfication
  • 2437578
• RefSeq identity
  • NM_001767
• Classification
  • CD molecules
  • Ig-like cell adhesion molecule family
  • C2-set domain containing
  • V-set domain containing
• VEGA ID
  OTTHUMG00000022750

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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