CD18 FITC, Single-Color Reagent
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Catalog number
0182
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Price
Please ask
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Size
100Tests
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Host Source
Mouse
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Clone Name
3EB
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Product Category
Flow Cytometry
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Species Reactivity
Human
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Application
Flow Cytometry
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Immunogen
NA
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Purification Method
Protein A/G Chromatography
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Shipping Conditions
Room Temperature
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Storage conditions
CD18 FITC, Single-Color Reagent should be stored at 4-8ºC. DO NOT FREEZE
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Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
ITGB2
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Short name
CD18 FITC, Single-Color Reagent
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Technique
FITC, Fluorescein
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Isotype
IgG1
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Label
FITC
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Alternative name
CD18 fluorecein, Single-Color Reagent
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Alternative technique
fluorescine
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Gene info
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Identity
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Gene
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Long gene name
integrin subunit beta 2
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Synonyms gene
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Synonyms gene name
- integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit)
- integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- Integrin beta subunits
- Complement system regulators and receptors
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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