CD13 FITC, Single-Color Reagent
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Catalog number
0132
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Price
Please ask
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Size
100Tests
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Host Source
Mouse
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Clone Name
E735
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Product Category
Flow Cytometry
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Species Reactivity
Human
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Application
Flow Cytometry
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Immunogen
CD13=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c x C57BL16 hybrid mice immunized with KG-1a cell line.
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Purification Method
Protein A/G Chromatography
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Shipping Conditions
Room Temperature
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Storage conditions
CD13 FITC, Single-Color Reagent should be stored at 4-8ÂșC. DO NOT FREEZE
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Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
ANPEP
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Short name
CD13 FITC, Single-Color Reagent
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Technique
FITC, Fluorescein
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Isotype
IgG1
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Label
FITC
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Alternative name
CD13 fluorecein, Single-Color Reagent
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Alternative technique
fluorescine
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Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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