Kidney Western Blot, 14 species

Test

Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Description

Monoclonals are usually produced in mouse as host species and polyclonals in rabbit or goat. Multi species reactivity is achieved by selecting consensus epitopes when blasting the gene for a reactive, innugenic epitope that is the same in the different target species.

Identity

HGNC:10640
Gene

CXCL14
Long gene name

C-X-C motif chemokine ligand 14
Synonyms gene
- SCYB14
Synonyms gene name
- small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK)
- chemokine (C-X-C motif) ligand 14
Synonyms
- BRAK
- NJAC
- bolekine
- Kec
- MIP-2g
- BMAC
- KS1
Synonyms name
- breast and kidney
GenBank acession
- AF073957
Locus

5q31.1
Discovery year

1999-07-30
Entrez gene record

9547
Pubmed identfication
- 10049774
RefSeq identity
- NM_004887
Classification
- Chemokine ligands
VEGA ID

OTTHUMG00000129139

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data