FITC: Negative Control IgG1
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Catalog number
STECH-8100
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Price
Please ask
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Size
0.5 mL
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.
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Properties
This Supertechs Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Supertechs FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Short name
FITC: Negative Control IgG1
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Technique
Negative, Control, FITC, controls, Fluorescein, negatif
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Isotype
IgG1
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Label
FITC
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Alternative name
fluorecein: Negative reference IgG1
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Alternative technique
controls, fluorescine
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Tissue
control
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MeSH Data
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Name
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Concept
Scope note:
The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
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Tree numbers
- E01.370.225.500.620.670.520
- E01.370.225.750.600.670.520
- E05.200.500.620.670.520
- E05.200.750.600.670.520
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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