JunD Antibody (pSer255): FITC

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• Catalog number
  SPC-1395D-FITC
• Price:

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• Size
  100 µl

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• Target
  JunD (pSer255)
• Conjugate
  FITC
• Research Area s
  Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Domain Families, Hlh, Cancer, Tumor Biomarkers, Oncoproteins, Oncoprotein Transcription Factors, Cardiovascular System, Heart, Hypertrophy
• Alternative Name s
  JUND Antibody, JunD FL isoform Antibody, Activator protein 1 Antibody, AP 1 Antibody, JUND_HUMAN Antibody, Transcription factor jun D Antibody, Jun D Antibody, jun D proto oncogene Antibody, Transcription factor jun-D Antibody, AP1 Antibody
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Synthetic phospho-peptide from human JunD around the phosphorylation site of serine 255 (pSer255).
• Immunogen Species
  Human
• Accession Number
  NP_001273897.1
• Gene ID
  3727
• Swiss Prot
  P17535
• Applications
  WB, IHC, ELISA, IP
• Host Species
  Rabbit
• Species Reactivity
  Human, Mouse, Rat
• Antibody Dilution
  WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:10000); IP (Various dilutions); optimal dilutions for assays should be determined by the user.
• Purification
  Peptide Affinity Purified
• Storage Buffer
  PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
• Specificity
  Detects endogenous levels of JunD only when phosphorylated at serine 255 (pSer255).
• Storage Temperature
  -20ºC
• Shipping Temperature
  Blue Ice or 4ºC
• Certificate of Analysis
  A 1:500 dilution of SPC-1395 was sufficient for detection of JunD (pSer255) in 10 µg of human embryonic kidney epithelial cell line (hek293) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
• Cellular Localization
  Nucleus, Nuclear Chromatin Nucleus, Transcription Factor Ap-1 Complex, Nuclear Chromatin, Chromatin, Protein-DNA Complex
• Field of Use
  For in vitro research use only.
• Technical data
• SDS
• Gross Weight kg
  1.4
• Net Weight g
  0.1
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

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• Gene target
  JunD   pSer255  
• Short name
  JunD Antibody (pSer255): FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Isotype
  IgG
• Label
  FITC
• Alternative name
  JunD (antibody to-) (pSer255): fluorecein
• Alternative technique
  antibodies, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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